ANALYSIS OF POLYMORPHISM OF ALPHA S1 CASEIN OF SLOVAK PINZGAU CATTLE BY PCR-RFLP
Keywords:
cattle, PCR-RFLP, -S1 caseinAbstract
The work was oriented to identification of -s1 casein gene polymorphism and
analysis of genotype structure in population of Slovak Pinzgau cattle. The material
involved 93 cattle. Bovine genomic DNA was isolated by fenol-chlorophorm
deprotenization and ethanol precipitation and used in order to estimate -s1 casein
genotypes by means of PCR-RFLP method. The PCR products were digested with
MaeIII restriction enzyme. In the population included in the study there were
homozygote genotype BB (81 animals) and heterozygote genotype BC (12 animals).
Homozygote genotype CC has not been observed. In the total population of cattle
homozygotes BB – 0.871 were the most frequent, while BC – 0.129 were the least
frequent ones. This suggests a superiority of allele B – 0.9355.
References
Aleandai, R., Buttazzoni, L. G., Schneider, J. C., Caroli, A., Davoli, R.,
, The Effects of Milk Protein Polymorphisms on Milk Components and
Cheese-Producing Ability, J.Dairy Sci., 73, 241-255
Beja-Pereira, A., Luikart, G., England, P.R., Bradley, D.G., Jann, O.C.,
Bertorelle, G., Chamberlain, A.T., Nunes, T.P., Metodiev, S., Ferrand, N., Erhardt, G., 2003, Gene-culture coevolution between cattle milk protein genes and human
lactase genes, Nature Genetics, 35, 311 – 313
Bovenhius, H., Van Arendonk, J. A. M., Korver, S., 1992, Associations
between milk protein polymorphisms and milk production traits, J. Dairy Sci., 75,
-2559
Caroli, A., Chessa, S., Bolla, P., Budelli, E., Gandini, G. C., 2004, Genetic
structure of milk protein polymorphisms and effects on milk production traits in a
local dairy cattle, Journal of Animal Breeding and Genetics, 121, 119-127(9)
Ferretti, L., Leone, P., Sgaramella, U., 1990, Long range restriction
analysis of the bovine casein gene, Nuc. Acids Res., 18, 6829-6833
Grosclaude, F., Mahé, M.F., Mercier, J.C., 1974 Comparaison du
polymorphisme génétique des lactoprotéines du zébu et des bovins, Annales de
Génétique et de Sélection Animale, 6, 305-329
Koczan, D., Hobom, G., Seyfert, H.M., 1991, Genomic organization of the
bovine alpha-S1 casein gene, Nuc. Acids Res., 19, 5591-5596
Koczan, D., Hobom, G., Seyfert, H.M., 1993, Characterization of the
bovine S1-casein gene C allele based on a MaeIII polymorphism, Anim. Genet.,
, 74
Prinzenberg, E. M., Weimann, C., Brandt, H., Bennewitz, J., Kalm, E.,
Schwerin, M., Erhardt, G., 2003, Polymorphism of the bovine CSN1S1 promoter:
Linkage mapping, intragenic haplotypes, end effects on milk production traits, J.
Dairy Sci., 86, 2696-2705
Sadik, A. F., 1972, Genetic variants of caseins and beta -lactoglobulins in
Pinzgau cattle from the Carpathians, Tsitologiya i Genetika, 6, 561-562
Sambrook, J., Fritz, E. F., Maniatis, T., 1989, Molecular cloning: A
laboratory manual. 2nd ed., Cold Spring Harb. Lab. Press, USA.1989.
Womack, J. F., Threadgill, D. W., Moll, Y. D., Faber, L. K., Foreman, M.
L., Dietz, A. B., Tobin, T. C., Skow, L. C., Zneimer, S. M., Gallagher, D. S.,
Rogers, D. S., 1989, Syntenic mapping of 37 loci in cattle, Cytogenet. Cell Genet.,
, 1109.
