Researches Concerning Reference Values Assessment of Serum Biochemical Parameters in some Fish Species from Acipenseridae, Cyprinidae, Esocidae and Salmonidae Family

Authors

  • Marioara Nicula University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Sciences and Biotechnologies, 300645 Timisoara, 119 Aradului Way, România
  • Marian Bura University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Sciences and Biotechnologies, 300645 Timisoara, 119 Aradului Way, România
  • Eliza Simiz University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Sciences and Biotechnologies, 300645 Timisoara, 119 Aradului Way, România
  • Ioan Banatean-Dunea University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Sciences and Biotechnologies, 300645 Timisoara, 119 Aradului Way, România
  • Silvia Pătruică University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Sciences and Biotechnologies, 300645 Timisoara, 119 Aradului Way, România
  • Adela Marcu University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Sciences and Biotechnologies, 300645 Timisoara, 119 Aradului Way, România
  • Mihai Lunca University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Sciences and Biotechnologies, 300645 Timisoara, 119 Aradului Way, România
  • Zoltan Szelei University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Sciences and Biotechnologies, 300645 Timisoara, 119 Aradului Way, România

Keywords:

Acipenseridae, Cyiprinidae, Esocidae, Salmonidae family, biochemical serum parameters

Abstract

The purpose of this work was to assess reference values of serum biochemical indices (enzymes, metabolites and minerals) in some representative fish species belonging to Acipenseridae, Cyprinidae, Esocidae and Salmonidae family in order to establish a baseline data which will serve to monitoring nutritional-metabolic balance and healthy condition of these species from aquatic biocenosis or intensive exploitations. Serum samples were analyzed for glucose, total proteins, albumine, urea nitrogen, creatinine, uric acid, triglycerides, cholesterol, ALT, AST, GGT, alkaline phosphatase, amylase, total bilirubin, Ca, P, Mg, Fe, using a FullyVet automated chemical analyser. The obtained results were compared with those from other papers regarding serum biochemical profile of fresh water fish species. Serum biochemical reference intervals were as follows: glucose, 28.41±0.80–64.00±1.41 mgdL-1; total proteins, 2.78±0.21-4.05±0.20 gdL-1; albumine, 0.67±0.12–1.59±0.11 gdL-1; urea nitrogen, 12.16±0.78–18.30±0.27 mgdL-1; creatinine, 0.06±0.01–0.27±0.00 mgdL-1; uric acid, 1.00±0.00–1.66±0.09 mgdL-1; triglycerides, 46.00±1.29– 351.3±12.3 mgdL-1; cholesterol, 123.00±2.12–198.00±0.91±mgdL-1; ALT, 15.00±0.91–32.00±1.29 IU L-1; AST, 30.00±0.91–92.00±1.47 IU L-1; GGT, 4.00±0.00–6.33±0.46 IU L-1; alkaline phosphatase, 60.33±1.20–109.50±3.05 IU L-1; amylase, 28.08±0.93 – 36.00±0.91 IU L-1; total bilirubin, 0.00±0.00–0.03±0.00 mgdL-1; Ca, 7.63±0.40–12.36±0.50 mgdL-1; P, 11.83±0.35-30.48±0.26 mgdL-1; Mg, 1.80±0.14–3.88±0.21mgdL-1; Fe, 57.60±3.48–120.00± 1.08 mgdL-1. The wide intra- and interspecific variability of our data requires subsequent studies of the endo- and exogenous factors (living condition, season, age, gender, origin, breeding system, physiological and nutritional status, genetic of each individual, etc.) that can induce variations of the propound parameter.

References

Michael, J. Manuel, Debra, L., Miller, D.V.M., Anita Merrill, Hematologic and plasma biochemical values of healty hybrid tilapia (Oreochromis aureus x Oreochromis nilotica) maintained in a recirculating system, Journal of Zoo and Wildlife Medicine, 2007, 38(3), 420-424.

Congleton, J. L., Wagner, T., Blood-chemistry indicators of nutritional status and food intake in juvenile salmonids, J. Fish Biol., 2006, 69, 473-490.

Fisheries and Oceans Canada (DFO), 2004, Blood sampling of finfish. Canada Department of Fisheries and Oceans animal-user training template, Home page address:http://www.ccac.ca/en/CCAC.../4_Blood_Sampling_of_Finfish.pdf

Jirasek, J., Mares, J., Palikova, M., Hematological and Biochemical Indices of blood in Wels (Silurus glanis L.) from intensive aquaculture, Acta Vet. Brno, 1998, 67, 227-233.

Hrubec, T.C., Cardinante, J. L., Smith, S.A., Hematology and serum chemistry reference intervals for cultured tilapia (Oreochromis hybrid), Vet.Clin. Pathol., 2000, 29, 7-12.

Svetina, A., Matasin, E., Tofant A., Hematology and some blood chemical parameters of young carp till the age of three years, 2002, 50(4), 459-467.

Chen, C.Y., Wooster, G. A, Getschell, R.G., Bowser, P. A., Timmons, M. B., Blood chemistry of healthy, nephrocalcinosis-affected and ozone-trated tilapia in a recirculating system, with application of discriminant analysis, 2003, Aquaculture 218, 89-102.

Allen, F. Mensinger, Blood biochemistry of the oyster toadfish, Journal of Aquatic Animal Healt, 2005, 17, 170-176.

Aras, M., Bayr, B., Sirkecioglu, A. N., Polat, H., Bayir, M., Seasonal variations in serum lipids, lipoproteins and some haematological parameters of chub (Leuciscus cephalus), Ital. J. Anim. Sci., 2008, 7, 439-448.

Satoh, S., Takanezawa, M., Akimoto, A., Kiron, V. and Watanabe, T., Changes of phosphorus absorption from several feed ingredients in rainbow trout during growing stages of extrusion of soybean meal. Fish. Sci. 2002, 68, 325–331.

Manera, M., Britti, D., Assessment of blood chemistry normal ranges in rainbow trout, Journal of Fish Biology, 2006, 69, 1427–1434.

Walsh, P.J., Mayer, G. D., Medina, M., Bernstein, M.L., Barimo, J. F., Mommsen, T.P., A second glutamine synthetase gene with expression in the gills of Gulf toarfish (Ospanus beta), Journal of Experimental Biology, 2003, 206, 1523-1533.

Wilson, D. W., Adolph, E. F. , The partition of non-protein nitrogen in the blood of fresh water fish, Journal of Biological Chemistry, 29 (3), 405-411.

Denis, W., The non-protein organic constituents in the blood of marine fish, J. Biol. Chem, 1922, 693-700

McCartney, T.H., Monthly variations of the serum total cholesterol of mature brown trout, Fisheries Research Bulletin of New York, 1966, 30, 42-45.

Munoz-Cueto, J. A., Alvarez, M., Blanco, M., Gonzales de Canales, M.L., Garcia-Garcia, A., Sarasquete, C., Histochemical and biochemical study of lipids during the reproductive cycle of the toad-fish, Halobatrachus didactylus (Schneider, 1801), Scientia Marina, 1996, 60, 289-296.

Wallaert, C., Babin, P. J., Age-related, sex related and seasonal changes of plasma lipoprotein concentrations in trout, J. Lipid Res., 1994, 35, 1619-1633.

Downloads

Published

2023-10-03