The Influence of Deuterium Depleted Water on the Hematocrit and the Leukocyte Formula in Rats Intoxicated With Chromium
Keywords:
chrome, deuterium depleted water, haematocrit, leukocyte formula, ratsAbstract
The aim of this study was to emphasize the influence of the deuterium-depleted water in the chromium (K2Cr2O7) single dose intoxication. The haematocrit and the leukocyte formula were determined, following the chrome intoxication in rats divided into 8 batches comprising 6 individuals each. The blood samples were collected directly from the heart (intracardiac punction) under narcosis. The data were processed statistically by means of the Kruskal-Wallis and Mann-Whitney tests, MINITAB 15 and SPSS17 Software. The haematocrit was improved and the non-specific defence realised by the neutrophils was stimulated constantly after the deuterium depleted water administration.
References
Trivedi, B., Saxena, D.K., Murthy, R.C., Chandra, S.V., Embriotoxicity and fetotoxicity of orally admistered hexavalent chromium in mice, Repro. Toxicol., 1989, 3, 4, 275-278.
Grande, S. W., Anderson, J. M., Talsness, CE, Grote, K, Chahoud, I, A dose – response study following utero and lactational exposure to Di(2-ethylhexyl)phthalate: Effects on female rat reproductive development, Toxicol Sci, 2006, 91, 1, 247-254.
Dayan, A.D., Paine, A.J., Mechanisms of chromium toxicity, carcinogenicity and allergenicity: review of the literature from 1985 to 2000, Human & Experimental Toxicology, 2001, 20, 9, 439–451, doi: 10.1191/096032701682693062.
De Mattia, G., Bravi, M. C., Laurenti, O, De Luca, O., Palmeri A., Sabatucci A., Mendico G., Ghiselli A., Impairment of cell and plasma redox state in subjects professionally exposed to chromium, 2004, American Journal of Industrial Medicine, 46, 2, 120-125.
Buzgariu, W., Caloianu, M., Lazăr, S., structural and ultrastructural changes in fish spleen induced by heavy water, 1997, Romanian Journal of Biological Science, 1, IX, 5-6.
Gyongyi Z., G. Somlyai, Deuterium depletion can decrease the expression of c-myc, Ha-Ras and p 53 gene in carcinoma treated mice, in vivo, 2000, 14, 3, 437-440.
Hăulica I., Peculea, M., Ştefănescu, I., Titescu, Gh., Todiraş, M., Bild, W., Effects of heavy and deuterium-depleted water of vascular reactivity, Romanian Journal of Physiology (Physiological Science), 1998, 35, 1-2, 25-32.
Haulica, I., Stefanescu, I., Bild, W., Titescu, Gh., Tamaian, R., Nastasa, V., Ionita, T., Research concerning physiological effects of deuterium - depleted water on unspecific immunity, 2003, http://www.europeea.ro
http://www.cdep.ro/pls/proiecte/upl_pck.proiect?idp=7433
http://www.ngo.ro/legislatie/index.shtml?AA_SL_Session=d94c4c70d3d7bb93b9d5942fd39b4ea7&x=7393
Ghergariu, S., Pop, Al., Kadar, L., Spînu, M., Manual de laborator clinic veterinar, Ed. All, Bucureşti, 2000, pp. 70-74.
Snyder, C.A., Udasin, I., Waterman, S.J., Taioli, E., Gochfeld, M., Reduced IL-6 levels among individuals in Hudson County, New Jersey, an area contaminated with chromium, Arch Environ Health., 1996, 5, 1, 26-28.
Sharma, B. K., P. C. Singhal, Chugh, K.S., Intravascular haemolysis and acute renal failure following potassium dichromate poisoning, Postgrad Med J., 1978, 54, 632, 414-415.
Glaser, U., Hochrainer, D., Klöppel, H., Kuhnen, H., Low level chromium (VI) inhalation effects on alveolar macrophages and immune functions in Wistar rats, Archives of Toxicology, 1985, 57, 4, 250-256, DOI 10.1007/BF00324787.
