Study of Different Types of Milk Protein Analysis Using Reverse Phase Chromatography RP-HPLC

Authors

  • Camelia Hodoşan University of Agronomical Sciences and Veterinary Medicine, 011464, Bucharest Blvd. Marasti 59 Romania
  • Lucica Nistor University of Agronomical Sciences and Veterinary Medicine, 011464, Bucharest Blvd. Marasti 59 Romania
  • Gratziela Buhaciu University of Agronomical Sciences and Veterinary Medicine, 011464, Bucharest Blvd. Marasti 59 Romania
  • Daniela Ianițchi University of Agronomical Sciences and Veterinary Medicine, 011464, Bucharest Blvd. Marasti 59 Romania
  • Florența Vilău
  • Ioana Dușescu SC HAAS Impex SRL, 100002, Ploiesti, Lapusna no.1, Romania

Keywords:

chromatography RP-HPLC, milk proteins

Abstract

Milk proteins have been extensively studied for over 90 years. Proteomics and associated technologies have the potential to facilitate an advanced knowledge of the proteins in milk. Proteomics allows detection, identification and characterization of milk proteins. In the present paper a study of milk proteins, using different analytical techniques - methods of dosing photometric and chromatographic analysis. With these techniques we want to reveal differences that occur at the protein level, between different animal species. As biological material was examined 4 different types of milk used for human consumption – cow’s milk, buffalo, sheep goats respectively.

References

Ferreira I., Cacote H., Detection and quantification of bovine, ovine and caprine milk percentages in protected denomination of origin cheeses by RP-HPLC og beta-lacto globulins, J. Chromatography A, 1015, 2003, 111-118

Bradford M., A rapid and sensitive method for the quantisation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72, 1976, 248-54.

Ciurdaru V., Andrei S., Pintea A., Bele C., Veterinary Medical Biochemistry - methods and laboratory techniques, Ed.AcademicPres, Cluj-Napoca 2001

Veloso A., Teixeira N., Ferreira I., Separation and quantification of the major casein fractions by RP-HPLC and urea-polyacrylamide gel electrophoresis – detection of milk adulterations, J. Chromatography A, 967, 2002, 209-218

Miranda G., Mahne M.F., Leroux C., Martin P., Proteomics tools to characterize the protein fraction of Equidae milk, Proteomics, 4, 2004, 2496-2509

Downloads

Published

2023-10-03

Issue

Section

Fundamental Sciences in Animal Husbandry