THE CONSEQUENCES ON BLOOD GSH DYNAMICS ON WISTAR FEMALE RATS AT AD LIBITUM CHROMIUM (VI) ADMINISTRATION DURING THE GESTATION AFTER THE WEAN
Keywords:
GSH, potassium dichromate, oxidative stress, female ratsAbstract
Chromium (VI) is a widely used industrial chemical, extensively used in paints,
metal finishes, steel including stainless steel manufacturing, alloy cast irons,
chrome, and wood treatment. In nature chromium occurs in divalent, trivalent and
hexavalent forms. Hexavalent chromium predominates over the trivalent form in
natural waters. We have studied the influence of potassium dichromate (K2Cr2O7)
on blood GSH values in rats. This study was carried out on 28 Wistar adult female
rats, divided in 3 experimental groups (E) and one control group (C). The rats
were feed with 25ppm (LOAEL), 50ppm and 75ppm potassium dichromate, ad
libitum, in drinking water, during the gestation. The control batch received tap
water. Reduced glutathione (GSH) was measured quantitatively after the wean
using a Perkin-Elmer spectrophotometer, through Beutler et al. method, at 412nm.
This study reports that potassium dichromate exposure induced the depletion of
blood GSH because Cr(VI) can generate reactive oxygen species (ROS). It can
induce oxidative stress and toxicity.
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