The Antioxidant Effect of a Functional Product Based on Probiotic Biomass, Pollen and Honey
Keywords:
probiotic, inulin, lactulose, antioxidantAbstract
The effect of prebiotics on the multiplication of some probiotic strains used in a pollen and honey medium was investigated in laboratory conditions, aiming to obtain a bee bread-like product. The experiment were carried out using six different bacterial species belonging to the genera Lactobacillus (four strains) and Bifidobacterium (two strains). The prebiotics inulin, lactulose and raffinose were tested and compared to honey. A productivity similar to that of honey resulted for inulin and lactulose. The medium based on pollen and honey was supplemented with the two prebiotics, the optimal values being obtained by using ground pollen and inulin, in the case of viability, as well as in the case of total antioxidant activity. After seven days of fermentation, the viability was over 300 CFU×106/ g and the total antioxidant activity was over 45%.
References
Bodera, P, Chcialowski, A., Immunomodulatory Effect of Probiotic Bacteria, Recent Pat Inflamm Allergy Drug Discov., 2009, 3, 1, 58-64.
Macfarlane, S., Steed, H., Macfarlane, G.T., Intestinal bacteria and inflammatory bowel disease, Crit Rev Clin Lab Sci., 2009, 46, 1, 25-54.
Kelly, G., Inulin-type prebiotics - a review: part 1, Altern Med Rev., 2008, 13, 4, 316-330.
Cavallini, D.C., Bedani, R, Bomdespacho, L.Q., Vendramini, R.C., Rossi, E.A., Effects of probiotic bacteria , isoflavones and simvastatin on lipid profile and atherosclerosis in cholesterol-fed rabbits: a randomized double-blind study, Lipids Health Dis., 2009, 8, 1, 1.
Bogdanov, S, Jurendic, T, Sieber, R, Gallmann, P., Honey for nutrition and health: a review, J Am Coll Nutr., 2008, 27, 6, 677-689.
Zeppa, G., Conterno, L., Gerbi, V., Determination of organic acids, glucides, diacetyl, and acetoin in cheese by high-performance liquid chromatography, J Agric Food Chem., 2008, 49, 6, 2722-2726.
Wang, H.F., Wang, Y.K., Yih, K.H., DPPH free-radical scavenging ability, total phenolic content, and chemical composition analysis of forty-five kinds of essential oils, J Cosmet Sci., 2008, 59, 6, 509-522.
Zarban, A., Ziaee, M., Evaluation of antioxidant properties of silymarin and its potential to inhibit peroxyl radicals in vitro,Pak. J. Pharm.Sci., 2008, 21, 3, 249-254.
Sasidharan, S., Darah, I., Mohd, J. N. M. K, Free radical Scavenging Activity and Total Phenolic Compounds of Gracilaria Changii, Int. J. of Nat. and Eng. Sci., 2007, 1, 3, 115-117