Identification of Enterococcus sp. in GIT of Broiler Chickens after Application of Biological Preparations
Keywords:
Enterococcus, chicken, detection, polymerase chain reactionAbstract
The aim of the present study was a rapid detection and identification of Enterococcus sp. in various segments of chicken gastrointestinal tract by polymerase chain reaction (PCR) analysis. As a biological material were used broiler chickens Hybro. They were fattening by the combined probiotic preparation for elimination of pathogens and better utilization of feed. In our study, the identification of Enterococcus species was based on the superoxid dismutase gene (sodA). Enterococcus faecium, Enterococcus faecalis were determined in all samples (100% occurence). Occurence of Enterococcus gallinarum was 87.5% and Enterococcus cecorum was 0%.
References
Ashayerizadeh, A., Dabiri, N., Ashayerizadeh, O., Mirzadeh, K.H., Roshanfekr, H, Effect of dietary antibiotic, probiotic and prebiotic as growth promoters, on growth performance, carcass characteristics and hematological indices of broiler chickens. Pakistan Journal of Biology Science 2009, 12, 52-57
Chichlowski, M., Croom, W.J., Edens, et al., Microarchitecture and spatial relationship between bacteria and ileal, cecal, and colonic epithelium in chicks fed a direct-fed microbial, primalac, and salinomycin, Poultry Science, 2007, 86, 1121-1132
Deasy, B. M., Rea, M. C., Fitzgerald, G. F., COGAN, T. M., Beresford, T. P., A rapid PCR based method to distinguish between Lactococcus and Enterococcus, Systematic Applied Microbiology, 23, 2000, 510–522
Devrise, L.A., Pot, B., Collins, M.D., Phenotypic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups, Journal of Applied Bacteriology, 1993, 75, pp. 399-408.
Franz, Ch., M., A., P., Holzapfel, H., Stiles, M., E., Enterococci at the crossroads of food safety?, Internat. Journal of Food Microbiology, 1999,47, 1–24
Haščík, P., Kačániová, M., Čuboň, J. Bobko, M., Nováková, I., et al, Application of Lactobacillus fermentum and its effect on chemical composition of Ross PM3 chicken meat, Acta fytotechnica et zootechnica (online), 2009, 12, 197 – 205
Haščík, P., Mihok, M., Kačániová, M., et al., Effect of multistrain probiotic application on changes in breast and thigh muscle of hybro broiler chickens post mortem, Potravinárstvo, 2010, 4,. 143 – 151
Higgins, J.P., Higgins, S.E., Vicente, J.L., Wolfenden, et al, Temporal effects of lactic acid bacteria probiotic culture on Salmonella in neonatal broilers, Poultry Science, 2007, 86, 1662-1666
Jin, L.Z.; Ho, Y.W.; et al., Growth performance, intestinal microbial populations and serum cholesterol of broilers fed diets containing Lactobacillus cultures. Poultry Science, 2008, 77, 1259-1265
Kabir, S.M.L., Rahman, M.M., Rahman, M.B., Hosain, M.Z., Akand, M.S.I., Das, S.K., Viability of probiotics in balancing intestinal flora and effecting histological changes of crop and caecal tissues of broilers, Biotechnology, 2005, 4, 325-330
Kabir, S.M.L., Rahman, M.M., Rahman, M.B., Rahman, M.M., Ahmed, S.U., The dynamics of probiotics on growth performance and immune response in broilers, International Journal of Poultry Science, 2004, 3, 361-364
Khaksefidi, A., Ghoorchi, T., Effect of probiotic on performance and immunocompetence in broiler chicks, Journal of Poultry Science, 2006, 43, pp. 296-300
Lu, J., Idris, U., Harmon, B., Hofacre, CH. et al., Diversity and succession of the intestinal bacterial community of the maturing broiler chicken. Applied and Enviromental Microbiology, 2003, 69, 6816-6824
Mountzouris, K.C., Tsirtsikos, P., Kalamara, E., Nitsch, S., Schatzmayr, G., Evaluation of the efficacy of probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poultry Science, 2007, 86, 309-317
Rada, V., Rychly, I., The effect of Lactobacillus salivarius administration on coliforms and enterococci in the crop and ceca of chicken broilers. Veterinary Medicine, 1995, 40, 311-315
Samanya, M., Yamauchi, K., Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. natto. Comparative Biochemistry and Physiology, 2002, 133, 95-104
Santos A.A., Poultry intestinal health through diet formulation and exogenous enzyme supplementation. Raleigh: North Carolina State University, 2005. 276p.
Tortuero, F. Influence of the implantation of Lactobacillus acidophilus in chicks on the growth, feed conversion, malabsorption of fats syndrome and intestinal flora. Poultry Science 2003, 52, 197-203
Zulkifli, I., Abdullah, N., Azrin, N.M., Ho, Y.W., Growth performance and immune response of two commercial broiler strains fed diets containing Lactobacillus cultures and oxytetracycline under heat stress conditions, British Poultry Science, 2000, 41, 593-597