The antibacterial effect assay of a few officinal plants
Keywords:
antibacterial assay, bacterial culture mediums, essential oils, inhibition areasAbstract
From very old times plants were used for man or animal health, with good results in slightly, functional injuries or in incipient stages of diseases. In chronic, when already appeared organic affection, officinal plants has an adjuvant part and can contributes to a partial reversibility of symptoms or lesions. This study was been performed using aqueous solutions or essential oils from Mentha spicata, Ocimum basilicum, Thymus vulgaris and Rosa sp. with bacterial cultures on usual or special culture mediums of Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica and Staphylococcus aureus. In view of antibacterial assay emphasizing were been used small pieces of absorbent paper immersed in solutions or essential oils above mentioned officinal plants. These pieces of papers then were been deposed in usual and special culture mediums containing the mentioned species of bacteria and then incubated 24 hours. The obtained result emphasized by the inhibition areas development recommend the antibacterial effect of essential oils of Rosa sp. (25.5 mm inhibition area), Mentha spicata (16.5 mm), Thymus vulgaris (7.3 mm) and Ocimum basilicum (5.1 mm) in therapeutic purpose, both in intern and extern way in infections caused by Bacillus cereus, Escherichia coli, Salmonella enterica and Staphylococcus aureus, in incipient stages.
References
Bojor, Ovidiu, Ghidul plantelor medicinale şi aromatice, ed. Fiat Lux, Bucureşti, 2003, pp. 161-185.
Bozin, B., Mimica-Dukic, N., Simin, N., Anackov, G., Characterization of the volatile composition of essential oils of some lamiaceae spices and the antimicrobial and antioxidant activities of the entire oils, J. Agric. Food Chem., 2006, 54(5), 1822-1828.
Brown, Deni, The Herb Society of America New Encyclopedia of Herbs & Their Uses, New York, D.K. pp. 120-283.
Ferro, V., A., Bradbury, F., Cameron, P., Shakir, E., Rahman, S., R., Stimson, W., H., In vitro susceptibilities of Shigella flexneri and Streptococcus pyogenes to inner gel of Aloe barbadensis Miller, J. Antimicrobial agents and chemotherapy, 2003, 47(3), 1137-1139.
Friedman, M., Henika, P., R., Mandrell, R., E., Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes and Salmonella enterica, 2002, J. Food. Prod., 65(10), 1545-1560.
Graedon, Joe, Theresa, Graedon, The People's Pharmacy Guide to Home and Herbal Remedies, ed. St. Martin's Griffin, New York, 2001, pp. 283-291.