Potassium Dichromate Impact on Male Reproductive Integrity Biomarker in Rat. Two Generation Study
Keywords:
chromium VI, histoarchitecture, male, rat, toxicityAbstract
The aim of this study was the evaluation of Cr VI cumulative and differentiate exposure on integrity biomarker of male reproductive system. The objective was evaluation of potassium dichromate intake on genital organs and sexual accessory glands architecture in male rats from two generation. Males and females from F0 generation were exposed to potassium dichromate three months before mating. F1 generation was represented by male pups, resulted from F0 generation, exposed to potassium dichromate in utero, during suckling (via milk and water) period and until sexual maturity. All the animals were divided in one control and three experimental groups, exposed to Cr VI trough drinking water, as followed E1: 25 ppm (LOAEL); E2: 50 ppm: E3: 75 ppm; control group received tap water without chromium content. The experiment was carried out with respecting legislation regarding ethics in animal experiments. The study revealed the presence of congestive and degenerative lesions in genital organs and sexual accessory glands of exposed individuals from F0 and F1 generation such as: interstitial edema, epithelial necrosis and atrophy, membrane exfoliation and necrosis. The lesions frequency and intensity were directly correlated to exposure level, the most affected being E3 group and generation, more pronounced in F1 generation.
References
De Celis R, Pedron-Nuevo N, Feria-Velasco A. Toxicology of male reproduction in animals and humans, Arch Androl 1996, 37, 201–18.
Agency for Toxic Substances and Disease Registry, Chromium Toxicity, 2001, Home page address: www.atsdr.cdc.gov
Zhitkovich A., Importance of chromium-DNA adducts in mutagenicity and toxicity of chromium (VI), Chemical Research in Toxicology 2005, 18, 3–11.
W. Mertz, Chromium in Human Nutrition: A Review, Journal of Nutrition, 1993, 123, 626–633.
Sugiyama, M., Role of physiological antioxidants in chromium (VI)-induced cellular injury, Free Radical Biol. Med. 1992, 12, 397–407.
World Health Organisation, Environmental Health Criteria, 61: Chromium. International Programme on Chemical Safety, Geneva, p. 197, 1988
Bagchi, D., Hassoun, A., Bagchi, M., Stohs, J., Chromium induced excretion of urinary lipid metabolites, DNA damage, nitric oxide production, and generation of reactive oxygen species in Sprague–Dawley rats, Comp. Biochemistry and Physiology, 1995, 2, 177–187
Saxena, D.K., Murthy, R.C., Lal, B., Srivastava, R.S., Chandra, S.V., Effect of hexavalent chromium on testicular maturation in the rat, Reproductive Toxicology, 1990, 4, 223–228.
Ernst, E., Testicular toxicity following short-term exposure to tri- and hexavalent chromium: an experimental study in the rat, Toxicology Letter, 1990, 51, 269–275.
Acharya, U.R., Mishra, M., Mishra, I., Tripathy, R.R., Potential role of vitamins in chromium induced spermatogenesis in Swiss mice, Environmental Toxicology and Pharmacology, 2004, 15, 53–59
Environmental Protection Agency, Locating and Estimating Air Emissions from Sources of Chromium, http://www.epa.gov
Chandra, A.K., Chatterjee, A., Ghosh, R., Sarkar, M., Chaube, S.K., Chromium induced testicular impairment in relation to adrenocortical activities in adult albino rats, Reproductive Toxicology, 2004, 24, 388-396
Chowdhury, A.R., Mitra, C. – Spermatogenic and steroidogenic impairment after chromium treatment in rats, Indian Journal of Experimental Biology, 1995, 33, 7, 480-484
Murthy, R.C., Saxena, D.K., Gupta, S.K., Chandra, S.V., Ultrastructural observations in testicular tissue of chromium-treated rats, Reproductive Toxicology, 1991, 5, 443-447
Muselin Florin – Impactul unor poluanţi asupra aparatului genital mascul şi calităţii materialului seminal, Teza de doctorat, 2006, USAMVB Timişoara
