Potassium Dichromate Impact on Sexual Cycle Duration and Regularity in Female Rats (F1 Generation)
Keywords:
female, F1, sexual cycle, ratAbstract
The study was carried out on 28 white Wistar adult female rats from F1 generation (derived from females and males exposed for three months before mating to potassium dichromate, 25, 50 and 75 ppm CrVI). F1 generation was exposed in utero and until sexual maturity to the same Cr VI levels. The study pointed out: significant increase of sexual cycles duration comparative to control group and over the physiological limits, directly correlated to exposure level (p<0.01); changes in regularity of sexual cycles, respectively: significant (p<0.01) decrease of proestrus, estrus and diestrus percentages in physiological limits as duration and inversely correlated to exposure level, appearance of sexual cycles with absent proestrus and estrus, directly correlated with the exposure level (p<0.01) and of prolonged diestrus, directly correlated to exposure level, (p<0.01).
References
Costa M., Klein, C. B., Toxicity and carcinogenicity of chromium compounds in humans, Crit. Rev. Toxicol., 36, 2006, pp.155-163;
Chiu, A., Katz, A. J., Beaubier, J., Chiu, N., Shi, X., Genetic and cellular mechanism in chromium and nickel carcinogenesis considering epidemiologic findings. Moll. Cell. Biochem., 255, 2004, pp. 181-194;
National Toxicology Program (2008ª) Toxicology and carcinogenesis studies of sodium dichromate dehydrate (CAS No.7789-12-0) in F344/N rats and B6C3F1 mice (drinking water studies). NTP technical report 546, NIH Publication 07-5887;
Toxicology Profile for Chromium, U.S. EPA, home page address: http://www.epa.gov, (2001);
Directiva 86/609 din 24.11.1986 privind pretecţia animalelor utilizate în scopuri experimentale şi în alte scopuri ştiinţifice
http://ec.europa.eu/food/fs/aw/aw_legislation/scientific/86-609-eec_en.pdf.;
Legea 205/26.05.2004 privind pretecţia animalelor, M. O. nr. 531/14.06.2004;
Legea 206/27.05.2004 privind buna conduită în cercetarea ştiinţifică, dezvoltarea tehnologică şi inovare, M. O. nr. 505/4.06.2004;
Legea 471/9.07.2002 privind aprobarea O.G. nr. 37/2002 pentru protecţia animalelor folosite în scopuri ştiinţifice sau în alte scopuri experimentale, M. O. nr. 535/23.07.2002;
Legea 9/11.01.2008 pentru modificarea şi completarea Legii nr. 205/2004 privind pretecţia animalelor, M. O. nr. 29/15.01.2008;
Ordin 143/400 pentru aprobarea instrucţiunilor privind adăpostirea şi îngrijirea animalelor folosite în scopuri ştiinţfice sau în alte scopuri experimentale, M. O. nr. 697/24.09.2002;
Kei-Ichiro Maeda., Satoshi Ohkura., Hiroko Tsukamura - Physiology of Reproduction, Academic Press, Japan, 2000, pp. 145-456,;
Junaid, M., Murthy, R. C. Saxena, D. K., Chromium fetotoxicity in mice during late pregnancy, Vet. Hum. Toxicol., 37(4), 2005, pp. 320-323;
Makar, R, Hexavalent Chromium in Drinking Water Causes Cancer in Lab Animals, 2007, home page adress:
http://www.nih.gov/news/pr/may2007/nies-16hm;
Rodriguez, R., Samuel, J., Arosh, J., Lee, J., Aruldas, M., Banu, S., Chromium toxicity induces ovarian follicular developmental arrest, apoptosis, and deregulated steroidogenesis: vitamin C restores follicular survival and function, Biology of Reproduction 77, 2007, pp. 215-215
