ASSESSMENT OF MOUSE EMBRYO VIABILITY BY ESTERASIC ACTIVITY DETECTION
Keywords:
embryo, viability, FDA, esterasic activityAbstract
In order to evaluate the esterasic activity within the viable embryos we used the
Fluorescein diacetate (FDA) staining test. For staining was used a 0.5 mg/ml FDA
stock solution. The embryos were recovered at 48 hours post coitus from
superovulated Swiss mouse females. Before staining the embryos were
microscopically evaluated by morphological criteria and classified in 4 quality
codes. The two methods used for quality and viability assessment were correlated
applying Pearson coefficient. The calculated value of the Pearson coefficient (r=1)
showed a strong correlation between the two methods used and indicate FDA
staining test and esterasic activity as a fast, easy and reliable method for embryo
viability assessment.
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