The Effect of Active Principles of Cilantro and Spirulina Powder on Lead Antagonism to Zinc and Iron in Carassius gibelio
Keywords:
fish, iron, lead intoxication, lyophilized cilantro, lyophilized spirulina, zincAbstract
Our research purpose was focused towards highlighting the lead toxicity on some tissues from Carassius gibelio Bloch (Prussian carp – Cyprinidae family) after induction of chronic intoxication with lead nitrate, hydrated with ½ water molecules – Pb(NO3)2x ½H2O. By this exploratory study, we wanted to research the detoxifying potential of the active principles from lyophilized cilantro and spirulina in experimental contamination with lead, to Carassius gibelio, and their effect on lead antagonism to iron and zinc.
120 Prussian carps, weighing 22-25 g each were divided according to the following treatments for 21 days: C group (without treatment), E1 group (75 ppm Pb into water as Pb(NO3)2x ½H2O), E2 group (75 ppm Pb into water+2% lyophilized cilantro in feed), E3 group (75 ppm Pb into water+2% lyophilized spirulina in feed).
At the end of the experimental period, tissue samples (gills, muscles myotome– epaxial, heart, skin and scales, intestine, liver, brain, gonads, kidney) were collected after a starving for 12 hours, and fish euthanasia with clove oil.
The analytical determination in order to establish zinc and iron concentrations, using atomic absorption spectrometer (AAS – VARIAN) was performed.
Cilantro and spirulina powder additionally administered in feed, has immobilized Pb through their active principles, thus decreasing its antagonistic effect expressed to Zn, even if Zn tissue level remained low. Pb antagonistic effect shown to Fe was diminished especially in tissue sampled from individuals receiving spirulina powder in feed.
References
Fish Feed Technology, http://www.fao.org.
Takeshi, W., Viswanath, K., Shuichi, S., Trace minerals in fish nutrition, Aquaculture, 1997, 151, 185-207.
Monisha Jaishankar, Tenzin Tseten, Naresh Anbalagan, Blessy B. Mathew, Krishnamurthy N. Beeregowda, Toxicity, mechanism and health effects of some heavy metals, Interdiscip Toxicol., 2014, 7(2), 60–72.
Flora, S. J. S., Mittal, M., Mehta, A., Heavy metal induced oxidative stress & its possible reversal by chelation therapy. Indian J Med Res., 2008, 128, 501–523.
Waldron, H. A., The Anaemia of Lead Poisoning: A Review, Br J Ind Med., 1966, 23(2), 83–100.
Aga, M., Iwaki, K., Ueda, Y., Ushio, S., Masaki, N., Fukuda, S., Kimoto, T., Ikeda, M., Kurimoto, M., Preventive effect of Coriandrum sativum (Chinese parsley) on localized lead deposition in ICR mice, Journal of Ethnopharmacology. 2001, 77(2-3), 203–208).
Upasani, C., Balaraman, R., Effect of vitamin E, vitamin C and spirulina on the levels of membrane bound enzymes and lipids in some organs of rats exposed to lead, Indian J. Pharmacol., 2001, 33, 185–191.
Amin, A., Hamza, A. A., Daoud, S., Hamza, W., Spirulina protects against cadmium-induced hepatotoxicity in rats, Am. J. Pharmacol. Toxicol., 2006, 1, 21–25.
Veena, B., Radhakrishnan, C. K. and Chacko, J., Heavy metal induced biochemical effects in an estuarine teleost, Indian J. Marine Sci., 1997, 26, 74-78.
Oprea, L. and Georgescu R., Nutritia si alimentatia pestilor, Ed. Colectia Fundatiei Universitare Dunarea de Jos, Galati, 2002.
Authman, M. M., Abbas, H. H., Accumulation and distribution of copper and zinc in both water and some vital tissues of two fish species (Tilapia zillii and Mugil cephalus) of Lake Qarun, Fayoum Province, Egypt., Pak J Biol Sci., 2007, 1, 10(13), 2106-22.
Hardy, R., W., Sullivan, C. V., Koziol, A. M., Absorption, body distribution, and excretion of dietary zinc by rainbow trout (Salmo gairdneri), Fish Physiol. Biochem., 1987, 3, 133-143.
Nutrient Requirements of Fish, National Academy Press Washington, D.C., 1993, pp.16-21
Shalaby, A. M., Protective effect of ascorbic acid against mercury intoxication in Nile tilapia (Oreochromius niloticus), J. Egypt. Acad. Soc. Environ. Develop., 2001, 2(3), 79-97.
Karuppasamy, R., Subathra, S., Puvaneswari, S., Haematological responses to exposure to sublethal concentration of cadmium in air breathing fish, Channa punctatus (Bloch), J. Environ Biol., 2005, 26(1),123-8.
Peraza, M. A., Ayala-Fierro, F., Barber, D. S., Casarez, E., Rael, L. T., Effects of micronutrients on metal toxicity, Environmental Health Perspectives, 1998, 106, Suppl 1. 203-216.
