The Effect of Active Principles of Cilantro and Spirulina Powder on Lead Antagonism to Copper and Chromium in Carassius gibelio
Keywords:
copper, chromium, fish, lead intoxication, lyophilized cilantro, lyophilized spirulinaAbstract
The goal of our work was to highlight the detoxifying potential of the active principles from lyophilized cilantro and spirulina in experimental contamination with lead, to Carassius gibelio, and their effect on lead antagonism to copper and chromium.
120 Prussian carps, weighing 22-25 g each were divided according to the following treatments for 21 days: C group (without treatment), E1 group (75 ppm Pb into water as Pb(NO3)2x ½H2O), E2 group (75 ppm Pb into water+2% lyophilized cilantro in feed), E3 group (75 ppm Pb into water+2% lyophilized spirulina in feed).
At the end of the experimental period, tissue samples (gills, muscles myotome– epaxial, heart, skin and scales, intestine, liver, brain, gonads, kidney) were collected after a starving for 12 hours, and fish euthanasia with clove oil.
Determination of Cu and Cr concentration in biological samples was performed using atomic absorption spectrophotometer AAS-VARIAN.
Pb addition into water in dose of 75 ppm, has resulted in Cu and Cr mobilization from fish tissues. Decreasing of Cu tissue level occurred less intensive in tissues sampled from groups receiving cilantro and spiriulina powder in feed, maximum efficiency in the counteracting the antagonism against Pb showing spirulina on the heart, liver, and kidney.
Cr was maintained at relatively low values, although, cilantro powder has induced in some wise the Pb complexing. In contrast, the freeze-dried spirulina brought the tissue level of Cr close to that of the control group or even has determined its more efficient takeover from the feed.
References
Fish Feed Technology, http://www.fao.org.
Takeshi, W., Viswanath, K., Shuichi, S., Trace minerals in fish nutrition, Aquaculture, 1997, 151, 185-207.
OECD, Risk Reduction Monograph No.1: Lead. OECD Environment Monograph Series No. 65. OECD Environment Directo-rate, Paris, France, 1993.
Landis, W. G., Ming-Ho, Y., Introduction to Environmental Toxicology: Impacts of Chemicals Upon Ecological Systems. CRC Press, Lewis Publishers, Boca Raton, Fl., 2003.
Blaurock-Busch, Chelation Therapy Handbook, https://microtrace.es/fileadmin/uploads/pdf/en/natural_chelators1.pdf.
Aga, M., Iwaki, K., Ueda, Y., Ushio, S., Masaki, N., Fukuda, S., Kimoto, T., Ikeda, M., Kurimoto, M., Preventive effect of Coriandrum sativum (Chinese parsley) on localized lead deposition in ICR mice, Journal of Ethnopharmacology. 2001, 77(2-3), 203–208.
Siva Kiran, R. R , Madhu, G. M, Satyanarayana, S. V., Spirulina in combating Protein Energy Malnutrition (PEM) and Protein Energy Wasting (PEW) - A review, Journal of Nutrition Research, 2016.
Ramakrishnan, C., M., Haniffa, M. A., Manohar, M., Effects of probiotics and spirulina on survival and growth of juvenile common carp (Cyprinus carpio), The Israeli Journal of Aquaculture – Bamidgeh, 2008, 60(2), 128–133.
Mustafa, Md. G., Umino, T., Nakagawa, H., The effect of Spirulina feeding on muscle protein deposition in red sea bream, Pagrus major, Journal of applied ichthyology, 1994, 10(2-3), 141–145.
Olvera‐Novoa, M. A.;, Dominguez‐Cen, L. J., Olivera‐Castillo, L., Martínez‐Palacios, Carlos A., Effect of the use of the microalga Spirulina maxima as fish meal replacement in diets for tilapia, Oreochromis mossambicus (Peters), fry.". Aquaculture research, 1998, 29 (10), 709–715).
Ali, Md. S., Evaluation of the effects of feed attractants (Spirulina and ekangi) on growth performance, feed utilization and body composition of fingerlings of stinging cat fish (Heteropneustes fossilis), 2014, http://repository.library.du.ac.bd/xmlui/handle/123456789/494?show=full.
Geffroy, B., Simon, O., Effects of a Spirulina platensis-based diet on zebra fish female reproductive performance and larval survival rate, Cybium, 2013, 37(1-2), 31–38.
Cuzon, G.; Santos, R. D.; Hew, M.; Poullaouec, G.,
Use of Spirulina in Shrimp (Penaeus japonicus) diet, J World Mariculture Society, 1981, 12(2), 282–291.
Nicula, M., Pacala, N., Stef L., Pet, I. Bencsik, I. Radulov I., Iancu T., Tulcan, C., Dragomirescu, M., Dronca, D., Berbecea, A., Dumitrescu G., Simiz E., Ahmadi, M. Marcu, A., The Effect of Active Principles of Cilantro and Spirulina Powder on Lead Antagonism to Zinc and Iron in Carassius gibelio, Animal Science and Biotechnologies, 2017, 50 (1), 265-271.
Josko O., and Sustar, N., Copper and Zinc, Biological Role and Significance of Copper/Zinc Imbalance, J Clinic Toxicol 2011, S: 3
Balk , E. M., Tatsioni, A., Lichenstein, A. H., Lau, J., Pitta, A. G., Effect of chromium supplementation on glucose metabolism and lipids: a systematic review of randomized controlled trials. Diabetes Care. 2007, 30(8), 2154-2163.
Bury, N. R., Walke,P. A., Glove, C. N., Nutritive metal uptake in teleost fish, The Journal of Experimental Biology, 2003, 206, 11-23.
Syed, L. S., Ahmet, A., Effects of heavy metal accumulation on the 96/h LC50 values in tench Tinca tinca L., 1758, Turk. J. Vet. Anim. Sci, 2005, 29, 139-144.
Silk, H. H., Axel, S., Wolfgang, E., James, H. M., Rainer, R. H. D., Thorsten, B., A respiratory hemocyanin from an insect, Proc Natl Acad Sci U S A. 2004, 2, 101(3), 871–874.
Brown, M., Harnessing chromium in the fight against diabetes,Drug Discovery today, 2003, 8, 962-963.
Kazim, S., Muhittin, O., Mehmet, T.,, Bilal, U., Gurkan, C., İbrahim, H. O., Vidyasagar, S., Vijaya J., James, R. K., Effect of chromium on carbohydrate and lipid metabolism in a rat model of type 2 diabetes mellitus: the fat-fed, streptozotocin-treated rat, Metabolism, 56(9), 2007, 1233-1240.
David, L. W., The Nutritional Relationships of Chromium, Journal of Orthomolecular Medicine, 1989, 4(1).
