Rapid Identification of Enterobacteriaceae in Milk and Dairy Products with the Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)
Keywords:
mass spectrometry, E. coli, Enterobacter, milk, dairy productsAbstract
Identification of microorganisms by MALDI-TOF MS Biotyper has been demonstrated to be accurate, rapid and lower cost than conventional food investigation methods. Rapid identification of pathogenic and spoilage microorganisms is crucial for dairy industry to ensure the quality and safety of milk and milk products. In this study, the bacterial species representing the Enterobacteriaceae family were identified in raw milk and milk products using the MALDI-TOF MS mass spectrometry. Altogether, 20 samples of Slovak milk and milk products were examined. Samples were cultured on VRBG agar at 37 °C for 24-48 h. Typical bacterial colonies were selected for identification with MALDI-TOF MS Biotyper. Escherichia coli and Enterobacter sp. were the most abundant Enterobacteriaceae family representatives identified. E. coli was found in nine and Enterobacter sp. in eight samples. Enterobacter sp. comprised 49 % and Escherichia coli 23 % of all bacterial isolates. The study shows that MALDI-TOF MS Biotyper was reliable identification method of Enterobacteriaceae in milk and dairy products. This method was helpful in evaluation of bacterial contamination of milk.
References
Mullane, N.R., Murray, J., Drudy, D., Prentice, N., Whyte, P., Wall, P.G., Parton, A., Fanning, S., Detection of Cronobacter sakazakii in dried infant milk formula by cationic-magnetic-bead capture. Applied Environmental Microbiology, 2006, 72, 6325–6330
Kornacki, J.L., Johnson, J.L., Enterobacteriaceae, coliforms, and Escherichia coli as quality and safety indicators. In: Downes P, Ito K (eds) Compendium of methods for the microbiological examination of foods, 4th edn. APHA, Washington, 2001, 69–82
Fox, A., Enterobacteriaceae, Vibrio, Campylobacter and Helicobacter, In Bacteriology – Chapter Eleven. Microbiology and Immunology on-line. University of South Caroline School of Medicine. 2010, http://pathmicro.med.sc.edu/fox/enterobact.htm
Holland, R.D., Wilkes, J.G., Rafii, F., Sutherland, J.B., Person, C.C., Voorhees, K.J., Lay, J.O., Rapid identification of intact whole bacteria based on spectral pattern using matrix-assisted laser desorption/ionization mass spectrometry, Rapid Commun Mass Spectrom, 1996, 10, 1227-12325. Krishnamurthy, T., Ross, P.L., Rapid identification of bacteria by direct matrix-assisted laser desorption ionization mass spectrometric analysis of whole cells, Rapid Commun Mass Spectrom, 1996, 10, 1992-1996
Bizzini, A., Durussel, C., Bille, J., Greub, G., Prod’hom G: Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory, Journal of Clinical Microbiology, 2010, 48, 1549-1554
Cherkaoui, A., Hibbs, J., Emonet, S., Tangomo, M., Girard, M., Francois, P., Schrenzel, J., Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level, Journal of Clinical Microbiology, 2010, 48, 1169-1175.
Seng, P., Drancourt, M., Gouriet, F., La Scola, B., Fournier, P.E., Rolain, J.M., Raoult, D., Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry, Clinical Infection Disease, 2009, 49, 543-551
van Veen, S.Q., Claas, E.C., Kuijper, E.J., High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories. Journal of Clinical Microbiology, 2010, 48, 900-907
Yamaguchi, N., Kitaguchi, A., Nasu, M. Selective enumeration of viable Enterobacteriaceae and Pseudomonas spp. in milk within 7 h by multicolor fluorescence in situ hybridization following microcolony formation, Journal of Bioscience and Bioengineering, 113(6), 746-750
Mormile, A., Scarano, L., Ariano, A., Murru, N., Vollano, L., Anastasio, A., Microbial characteristics of Conciato Romano: an artisanal cheese made from raw sheep’s milk, Italian Journal of Food Safety, 2013, 2(46), 170-172
Gerasi, E., Litopoulou-Tzanetaki, E., Tzanetakis, N., Microbiological study of Manura, a hard cheese made from raw ovine milk in the Greek island Sifnos, International Journal of Dairy Technology, 2003, 117, 122-32
Pisano, M.B., Fadda, M.E., Deplano, M., Corda, A.,
Cosentino, S., Microbiological and chemical characterization of Fiore Sardo, a traditional Sardinian cheese made from ewe’s milk, International Journal of Dairy Technology, 2006, 171, 179-89
Corbo, M.R., Lanciotti, R., Albenzio, M., Sinigaglia, M., Microbiology Occurrence and characterization of yeasts isolated from milks and dairy products of Apulia region, Internatinal Journal of Food Microbiology, 2001, 147, 152-69
Kagkli, D.M.M., Vancanneyt, P., Vandamme, CH., Cogan, T.M., Contamination of milk by enterococci and coliforms from bovine faeces, Journal of Applied Microbiology, 2006,1364-507
Jayarao, B.M., Wang, L., A study on the prevalence of Gram negative bacteria in bulk tank milk, Journal of Dairy Science, 1999, 82, 2620-2624
Ntuli, P., Njage, M.K., Buys, E.M., Characterization of Escherichia coli and other Enterobacteriaceae in producer-distributor bulk milk V, Journal of Dairy Science, 2016, 99, 9534–9549
Doulgeraki, A.I., Paramithiotis, S., Nychas, G.J.E.. Characterization of the Enterobacteriaceae community that developed during storage of minced beef under aerobic or modified atmosphere packaging conditions, International Journal of Food Microbiology, 2011, 145, 77–83
Miranda, J., Guarddon, M., Vázquez, B., Fente, C., Barros-Velazquez, J., Cepeda, A., Franco, C., Antimicrobial resistance in Enterobacteriaceae strains isolated from organic chicken, conventional chicken and conventional turkey meat: A comparative survey, Food Control, 2008, 19:412–416
Van Kessel, J., Karns, J., Gorski, L. McCluskey, B., Perdue, M. Prevalence of Salmonellae, Listeria monocytogenes, and fecal coliforms in bulk tank milk on US dairies, Journal of Dairy Science, 2004, 87, 2822–2830.
