Gross Morphology Analysis of Osteoarthritis in Rabbits
Keywords:
glucosamine sulfate, gross morphology, knee joint, osteoarthritis, rabbitAbstract
The aim of this study was to evaluate the effect of glucosamine sulfate on morphological changes investigated on the articular cartilage of knee joint during development of osteoarthritis in rabbits following collagenase-induced osteoarthritis. A total of 32 New Zealand White male and female rabbits were used in this study. All animals were kept in standard conditions at Research Institute of Livestock Production in Nitra. Animals were divided into 4 groups: healthy control group, osteoarthritic untreated group and two experimental groups. After collagenase-induced osteoarthritis daily oral administration of glucosamine sulfate ( 80 mg.kg-1 and 160mg.kg-1 of live weight) was performed. At the end of the eight week, the rabbits were sacrificed and their both right and left knees with proximal femur and distal tibia were harvested. Gross morphology of knee joint was tested. Basal statistical indicators were calculated from obtained data using Statgraphics software and analysis of variance. Morphological changes in osteoarthritic group without treatment were seen on both, medial and lateral region, but markedly on medial condyle. Articular cartilage was characterized by a rough and hypertrophic appearance with severe erosions. The severity of cartilage damage was generally lower in glucosamine treated groups in comparison with osteoarthritis group. The gross morphological examination of healthy control revealed very little or no changes. Gross morphological grading of cartilage damage showed significantly lower extent of damage in glucosamine treated groups.
References
Dungl, P., Ortopedie. Orthopedie, Czech lang., Praha: Grada, 2005, 80-247-0550-8.
Trnavský, K., Osteoartróza. Osteoarthritis,. slovak lang Praha: Galén, 2006.
Reeve, J. Bone, Structural changes and hip osteoarthritis: Comment on the article by Javaid et al. Arthritis Rheum., 2010, 62(3), 909-10.
Kuettner, K. E, Cole, A. A., Cartilage degeneration in different human joints. Osteoarthritis Cartilage, 2005, 13(2), 93-103.
Persiani, S., Roda, E., Rovati, L. C., Glucosamine oral bioavailability and plasma pharmacokinetics after increasing doses of crystalline glucosamine sulfate in man. Osteoarthritis Cartilage, 2005 a, 13(12), 1041-49.
Kim J, Chung J, Ok, H., Asymptomatic acromioclavicular joint arthritis in arthroscopic rotator cuff tendon repair: a prospective randomized comparison study. Arch Orthop Trauma Surg., 2011, 131(3), 363-9. doi: 10.1007/s00402-010-1216-y.
Pastoureau, P. C, Chomel, A. C, Bonnet, J., Evidence of early subchondral bone changes in the menisectomized Guinea pig. A densitometric study using dual energy X-ray absorptiometry subregional analysis, Osteoarthritis Cartilage, 1999, 7(5), 466-73.
Pastoureau, P. C, Chomel, A. C., Methods for cartilage and subchondral bone histomorphometry, Methods Mol Med., 2004, 10(1), 79-91.
Settle, S, Vickery, L., Nemirovskiy, O., Cartilage degradation biomarkers predict efficacy of a novel, higly selective matrix metalloproteinase 13 inhibitor in a dog model of osteoarthritis: confirmation by multivariate analysis that modulation of type II collagen and aggrecan degradation peptides parallels pathologic changes., Arthritis Rheum., 2010, 62(10), 3006-15.
Šteňo, B., Šeliga. J., Farmakologická liečba osteoartrózy, Pharmacological treatment of osteoarthritis,. slovak lang, Ambulantná terapia., 2008, 6 (3), 164-8.
Richette, P., Effect of hyaluronic acid in symptomatic hip osteoarthritis, Arthritis Rheum., 2009 60 (3) 824-30.
Persiani, S., Roda, E., Rovati, L. C., Locatelli, M., Giacovelli, G, Roda, A.. Glucosamine plasma and synovial fluid concentrations before and after oral administration of crystalline glucosamine sulfate in knee osteoarthritis patients, Arthritis Rheum., 2005 b, 502-08.
Setnikar, I., Palumbo, R., Pharmakokinetics of glucosamine in man. Arzneimittelforschung/Drug Res., 1993, 43(2), 1109-13.
Clegg, D. O., Reda, J., Glucosamine, chondroitin sulfate and the two in combination for painful knee osteoarthritis, N Engl J Med., 2006, (8), 795-08.
Bigee, B. A., McAlindon, T., Glucosamine for osteoarthritis: part II, biologic and metabolic controversies, Med Health R I., 2004, 87(6), 180-1.
Burr, D. B, Radin, E. L., Microfractures and microcracks in subchondral bone: are they relevant to osteoarthrosis?, Rheum Dis Clin North Am., 2003, (4), 675-85.
Ebube, N. K., Mark, W., Hahm, H., Preformulation studies and characterization of proposed chondroprotective agents: glucosamine HCl and chondroitin sulfate, Pharm Dev Technol., 2002, 7 (4), 457-69.
Lammi, M., Does oral glucosamine prevent the loss of proteoglycans in a rabbit model of osteoarthritis?, Arthritis Rheum., 2005, 52 (11), 3680-81. PMID:16258912
