CHANGES INDUCED BY SOME NUTRITIONAL FACTORS ON TIBIAL BONE STRUCTURE IN PIG

GABI DUMITRESCU, LAVINIA STEF, D. DRINCEANU, LILIANA PETCULESCU CIOCHINA, D. STEF, D. DRONCA, RODICA CRISTE, LILIANA BOCA, C. JULEAN

Abstract


The problems approached by our team are represented by the involvement of different sources and levels of calcium, namely calcium carbonate, fructoborate and alfalfa, in the mineralization of bone tissue. So, we made fix histological preparations of tissue fragments taken from tibia bone, from 9 pigs belonging to three batches: the control batch, where calcium was provided in a proportion of 1% through calcium carbonate, experimental batch 1, where calcium was provided in a proportion of 1.04% through fructoborate, on a calcium carbonate support, and experimental batch 2, where calcium was provided in a proportion of 1.13% through fructoborate + alfalfa, on a calcium carbonate support. The histomorphometric parameters assessed were represented by the volume of bone trabeculae (BV/TV, %) or the percentage of bone tissue in a given volume and the mean width of bone trabeculae. At the same time, in order to establish fructoborate and alfalfa implication in bone mineralization, we supervised the presence and activity of osteoblasts respectively osteoclasts. In the case of the experimental batch 1, the histomorphometric study shows an increase of bone trabeculae dimension, with a mean width of about 142,5μ μ, and also an increase of their mean volume, which is about 37,11%. The trabecular system is dense and present mineralised and ossified territory where are formed by osteoclasts with osteocytes. Peritrabeculary are presented active osteoblasts which are involved in plurilamellar stratification by deposition of young collagen. In the case of individuals from experimental batch 2, trabeculae mean width is about 140,7μ, while their volume is 35,62%.


Keywords


pig; bone; fructoborate; calcium; histomorphometry

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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Faculty of Animal Science and Biotechnologies
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
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