Polymorphism within the Intron Region of the Bovine Leptin Gene in Slovak Pinzgau Cattle

Nina Moravčíková, Anna Trakovická, Radovan Kasarda

Abstract


Leptin is 16 kDa protein synthesized by adipose tissue and involved energy balance by inhibiting food intake and stimulating energy expenditure, reproductive and immune functions. The physiological properties support leptin as a strong candidate gene for evaluating of genetic polymorphism, which has further been associated with growth, milk yield and other economical traits in livestock. The aim of this study was detection polymorphism in the intron region of the leptin gene on bovine chromosome 4 by using PCR-RFLP method. A total of 100 Slovak Pinzgau cows were used for SNP genotyping. A strategy employing PCR was used to amplify a 422 bp product from hair roots DNA samples. The genotype and allele frequencies for each breed were determined and tested to be in Hardy-Weinberg equilibrium. Digestion of PCR products with restriction enzyme Sau3AI revealed two alleles: allele A was 390, 32 fragments and allele B was 303, 88, 32. Three patterns were observed and frequencies were 0.4761, 0.4278 and 0.0961 for AA, AB and BB, respectively.


Keywords


cattle; leptin gene; PCR-RFLP; polymorphism

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Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Faculty of Animal Science and Biotechnologies
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
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