Practical Methods to Assess Mammalian Embryo Quality – Staining Tests Comparative Study

Alexandra Ivan, Nicolae Păcală, Ada Cean, Valeriu Carabă

Abstract


Embryo assessment methods changed during the last 20 years. Morphological criteria used for embryo quality assessment improved in order to increase the selection probability of the most suitable embryos for transfer. This paper is presenting, based on a rich practical experience cumulated in our research group, several practical methods used for embryo assessment. For this study we have comparative evaluate the embryo quality using four staining tests: Trypan blue staining, Neutral red staining, Fluorescein diacetate staining, Propidium iodide staining in order to establish which test is the most reliable and efficient in embryo quality evaluation. The results obtained indicated that the Fluorescein diacetate and Propidium iodide viability test can be successfully used for mouse embryo viability evaluation. This method allows detection of both viable and nonviable cells.


Keywords


mouse embryo; quality; staining tests

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Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Faculty of Animal Science and Biotechnologies
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110