The Use of High Resolution Melting Analysis for Detection of the CAPN530 Polymorphism in Slovak Simmental Bulls

Michal Gábor, Anna Trakovická, Martina Miluchová, Nikola Kleknerová

Abstract


The high-resolution melting analysis is a cost-effective and simple post-PCR technique that can be used for SNPgenotyping. The objective of this study was to apply the high resolution melting analysis for detection of SNPpolymorphism CAPN530 of bovine calpain gene (CAPN1) in Simmental bulls. A total 42 Simmental bulls wereincluded in this study. Genomic DNA was extracted from sperm by commercial Nucleospin Tissue kit. Theamplification and high resolution melting analysis (HRMA) was done by commercial SensiMixTM HRM kit in thereal-time thermocycler Rotor-Gene 6000. The confirmation of sensitivity of this method was done by PCR-RFLPmethod. In the analyzed population of Simmental bulls were detected the following frequencies of alleles andgenotypes for the SNP CAPN530 of the CAPN1 gene. Frequencies of allele A and allele G were 0.3929 and 0.6071and frequencies of genotypes were 0.1191 (genotype AA), 0.5476 (genotype AG) and 0.3333(genotype GG).

Keywords


cattle; genomic DNA; high-resolution melting analysis (HRMA); SNP polymorphism CAPN530

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Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

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