Genetic Structure of Alpha S1 Casein in Slovak Pinzgau Cattle

Anna Trakovická, Michal Gábor

Abstract


The work was oriented to identification of -s1 casein gene polymorphism and analysis of genotype structure in population of Slovak Pinzgau cattle. The material involved 39 cattle. Bovine genomic DNA was isolated by commercial kit NucleoSpin Blood (Macherey-Nagel) and ethanol precipitation and used in order to estimate -s1 casein genotypes by means of PCR-RFLP method. The PCR products were digested with MaeIII restriction enzyme. In the population included in the study, homozygote genotype BB (39 animals) and heterozygote genotype BC (3 animals). Homozygote genotype CC has not been observed. In the total population of cattle homozygotes BB – 0.9231 were the most frequent, while BC – 0.0769 were the least frequent ones. This suggests a superiority of allele B – 0.9615.


Keywords


-S1 casein; cattle; PCR-RFLP

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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Faculty of Animal Science and Biotechnologies
300645, Timişoara, Calea Aradului 119, Romania

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