HinfI polymorphisms characterisation in the 1st intron of BMP15/FecX gene in Turcana and Tsigai sheep (Ovis aries)

Viorica Coşier

Abstract


BMP15-FecX is one of the major genes associated with prolificacy in different sheep breeds (Ovis aries), alongside BMPR15/FecB and GDF9/FecG. The majority of the polymorphisms, associated with ovulation rate in different breeds, are located in the exonic sequences of the gene. In the present study, a fragment of 356 bp, adjacent to the 2nd exon of the BMP-15 gene, was amplified by PCR in 50 individuals of Turcana and Tsigai breeds. The amplified products were subjected to digestion to identify the polymorphisms in the HinfI restriction enzyme sites. The two putative enzyme sites presented in the fragment did not reveal mutations in neither breed, releasing the same electrophoretic profile.

Keywords


SNP, Polymorphism, BMP15 Gene, Restriction enzyme

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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

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