In vitro evaluation of some probiotic properties of Lactobacillus strains isolated from chickens’ gut

Mihaela Dumitru, Mihaela Dumitru, Georgeta Ciurescu, Ionut Sorescu

Abstract


The objective of this study was to isolate, identify, and characterize new Lactobacillus strains with high probiotic potentials from the gastrointestinal tract of broiler chicks’. The selected lactobacilli include five strains as fallow Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus fermentum, Lactobacillus salivarius and Lactobacillus plantarum. All strains were evaluated for their viability on selective medium, pH 3.0 tolerance and 0.3% bile salts for 3h. The strains IBNA 29 and IBNA 48 were the most resistant following exposure to gut conditions. After 3h of incubation, IBNA 29 registered a hight survival rate around 85% at low pH (8.50±0.176 log CFU/mL), respectively 90% (9.01±0.045 log CFU/mL) in the presence of bile salts; IBNA 48 showed growth with 15.32% (8.027±0.04 CFU/mL), and 34.32% (6.22±0.242 CFU/mL), less at these useful traits. Taken together, the strain Lactobacillus salivarius IBNA 29 manifested the best resistance with the potential for use in the production of probiotic and can be a possible candidate for application as a feed additive in poultry nutrition.


Keywords


gastrointestinal resistance, in vitro properties, probiotic

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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
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