Antimicrobial Effect of Lactobacillus kunkeei Against Pathogenic Bacteria Isolated from Bees’ Gut

Miroslava Kačániová, Simona Kunová, Eva Ivanišová, Margarita Terentjeva, Jaroslav Gasper


The aim of this study was to evaluate antimicrobial activity of bee’s gastrointestinal tract (GUT) Lactobacillus kunkeei against pathogenic bacteria isolated from bees GUT. Content of the intestinal tracts microflora were cultured for isolation of different group of bacteria. Gut homogenates were plated on de Man, Rogosa and Sharpe agar plates and incubated for 48-72h at 30 °C anaerobically and McConkey agar and incubated for 24-48h at 37°C. Then, the identification of isolates with MALDI-TOF MS Biotyper was done. The bacterial strains Lactobacillus kunkeei, Bacillus cereus, Bacillus megatherium, Bacillus simplex, Bacillus thuringiensis, Delfia acidovorans, Escherichia coli, Enterococcus faecalis, Klebsiella aerogenes, Morganella morganii, Pseudomonas aeruginosa, Serratia marcescens and Sphingomonas parapaucimobilis, were isolated from gut content of bees. The disc diffusion method was used for the determination of antimicrobial activities of the Lactobacillus kunkeei supernatant against other isolated bacterial strains from GUT bees. The best antimicrobial activity of Lactobacillus kunkeei supernatant against Klebsiella aerogenes, Morganella morganii, Pseudomonas aeruginosa, Serratia marcescens. Lesser degree of antimicrobial activity against Bacillus cereus, Bacillus megatherium, Bacillus simplex, Bacillus thuringiensis were found in L. kunkeei supernatant. The moderate antibacterial activity against Delfia acidovorans, Escherichia coli, Enterococcus faecalis, Sphingomonas parapaucimobilis were found in L. kunkeei supernatant.


Lactobacillus kunkeei, antimicrobial activity, bees, pathogenic bacteria

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