Antimicrobial Effect of Lactobacillus kunkeei Against Pathogenic Bacteria Isolated from Bees’ Gut

Miroslava Kačániová, Simona Kunová, Eva Ivanišová, Margarita Terentjeva, Jaroslav Gasper

Abstract


The aim of this study was to evaluate antimicrobial activity of bee’s gastrointestinal tract (GUT) Lactobacillus kunkeei against pathogenic bacteria isolated from bees GUT. Content of the intestinal tracts microflora were cultured for isolation of different group of bacteria. Gut homogenates were plated on de Man, Rogosa and Sharpe agar plates and incubated for 48-72h at 30 °C anaerobically and McConkey agar and incubated for 24-48h at 37°C. Then, the identification of isolates with MALDI-TOF MS Biotyper was done. The bacterial strains Lactobacillus kunkeei, Bacillus cereus, Bacillus megatherium, Bacillus simplex, Bacillus thuringiensis, Delfia acidovorans, Escherichia coli, Enterococcus faecalis, Klebsiella aerogenes, Morganella morganii, Pseudomonas aeruginosa, Serratia marcescens and Sphingomonas parapaucimobilis, were isolated from gut content of bees. The disc diffusion method was used for the determination of antimicrobial activities of the Lactobacillus kunkeei supernatant against other isolated bacterial strains from GUT bees. The best antimicrobial activity of Lactobacillus kunkeei supernatant against Klebsiella aerogenes, Morganella morganii, Pseudomonas aeruginosa, Serratia marcescens. Lesser degree of antimicrobial activity against Bacillus cereus, Bacillus megatherium, Bacillus simplex, Bacillus thuringiensis were found in L. kunkeei supernatant. The moderate antibacterial activity against Delfia acidovorans, Escherichia coli, Enterococcus faecalis, Sphingomonas parapaucimobilis were found in L. kunkeei supernatant.


Keywords


Lactobacillus kunkeei, antimicrobial activity, bees, pathogenic bacteria

Full Text:

PDF

References


vanEngelsdorp, D., Evans, J.D., Donovall, L., Mullin, C., Frazier, M., Tarpy, D.R., Hayes, J., Pettis, J.S., Entombed Pollen”: A new condition in honey bee colonies associated with increased risk of colony mortality, Journal of Invertebrate Pathology, 2009, 101, 147-149

Mullin, C.A., Frazier, M., Frazier, J.L., Ashcraft, S., Simonds, R., vanEngelsdorp, D., Pettis, S., High levels of miticides and agrochemicals in North merican apiaries: implications for honey bee health, PLoS One, 2010, 5, e9754

Gilliam, M., Valentine, D.K., Enterobacteriaceae isolated from foraging worker honey bees, Apis mellifera, Journal of Invertebrate Pathology, 1974, 23, 38-41

Pettis, J.S., vanEngelsdorp, D., Johnson, J., Dively, G., Pesticide exposure in honey bees results in increased levels of the gut pathogen Nosema, Naturwissenschaften, 2012, 99, 153-158

Pettis, J.S., Lichtenberg, E.M., Andree, M., Stitzinger, J., Rose, R., vanEngelsdorp, D., Crop pollination exposes honey bees to pesticides which alters their susceptibility to the gut pathogen Nosema ceranae, PLoS One, 2013, 8, e70182

Runckel, C., Flenniken, M.L., Engel, J.C., Ruby, J.G., Ganem, D., Andino, R., DeRisi, J.L., Temporal analysis of the honey bee microbiome reveals four novel viruses and seasonal prevalence of known viruses, Nosema, and Crithidia, PLoS One 2011, 6, e20656

Singh, R., Levitt, A.L., Rajotte, E.G., Holmes, E.C., Ostiguy, N., vanEngelsdorp, D., Lipkin, W.I., dePamphilis, C.W., Toth, A.L., Cox-Foster, D.L., RNA Viruses in Hymenopteran Pollinators: Evidence of Inter-Taxa Virus Transmission via Pollen and Potential Impact on Non-Apis Hymenopteran Species, PLoS One 2010, 5, e14357

Cornman, R.S., Tarpy, D.R., Chen, Y., Jeffreys, L., Lopez, D., Pettis, J.S., vanEngelsdorp, D., Evans, J.D., Pathogen webs in collapsing honey bee colonies, PLoS One, 2012, 7, e43562

Ravoet, J., Maharramov, J., Meeus, I., De Smet, L., Wenseleers, T., Smagghe, G., de Graaf, D.C., Comprehensive Bee Pathogen Screening in Belgium Reveals Crithidia mellificae as a New Contributory Factor to Winter Mortality, PLoS One 2013, 8, e72443

Dainat, B., Evans, J.D., Chen, Y.P., Gauthier, L., Neumann, P. Predictive markers of honey bee colony collapse, PLoS One, 2012, 7, e32151

Hamdi, C., Balloi, A., Essanaa, J., Crotti, E., Gonella, E., Raddadi, I., Ricci, A., Boudabous, S., Borin, A., Manino, C., Bandi, A., Alma, D., Daffonchio, Cherif, A. Gut microbiome dysbiosis and honeybee health, Journal of Applied Entomology, 2011, 135, 524-533

Anderson, K.E., Sheehan, T.H., Eckholm, B.J., Mott, B.M., DeGrandi-Hoffman, G., An emerging paradigm of colony health: microbial balance of the honey bee and hive (Apis mellifera), Insectes Society, 2011, 58, 431-444

Gilliam, M., Identification and roles of non-pathogenic microflora associated with honey bees FEMS Microbiology Letters, 1997, 155, 1-10

Forsgren, E., Olofsson, T.C., Vásquez, A., Fries, I. Novel lactic acid bacteria inhibiting Paenibacillus larvae in honey bee larvae, Apidologie, 2010, 41, 99-108

Vasquez, A., Forsgren, E., Fries, I., Paxton, R.J., Flaberg, E., Szekely, L., Olofsson, T.C. Symbionts as major modulators of insect health: lactic acid bacteria and honeybees, PLoS One, 2012, 7, e33188

Olofsson, T.C., Butler, E., Markowicz, P., Lindholm, C., Larsson, L., Vasquez, A., 2014. Lactic acid bacterial symbionts in honeybees-an unknown key to honey's antimicrobial and therapeutic activities, International Wound Journal, 10.1111/iwj.12345

Butler, E., Alsterfjord, M., Olofsson, T.C., Karlsson, C., Malmström, J., Vásquez, A., Proteins of novel lactic acid bacteria from Apis mellifera: an insight into the production of known extra-cellular proteins during microbial stress, BMC Microbiology, 2013, 13, 235

Butler, E., Oien, R.F., Lindholm, C., Olofsson, T.C., Nilson, B., Vásquez, A., A pilot study investigating lactic acid bacterial symbionts from the honeybee in inhibiting human chronic wound pathogens, International Wound Journal, 2014, doi: 10.1111/iwj.12360

Nousiainen, J., Javanainen, P., Setala, J., Wright, A.V., Lactic acid bacteria as animal probiotics, Salminen, S., Wright, A.V., Ouwehand, A., (Eds.), Lactic Acid Bacteria Microbiological and Functional Aspects. Edited by A, Marcel Dekker, Inc., New York 2004, 547-580

Vásquez, A., Forsgren, E., Fries, I., Paxton, R.J., Flaberg, E., Szekely, L., Olofsson, T.C., Symbionts as major modulators of insect health: lactic acid bacteria and honeybees, PLoS One, 2012, 7, e33188

Endo, A., Irisawa, T., Futagawa-Endo, Y., Takano, K., du Toit, M., Okada, S., Dicks, L.M.T., Characterization and emended description of Lactobacillus kunkeei as a fructophilic lactic acid bacterium, International Journal of Systematic Evolutionary Microbiology, 2012, 62, 500-504

Edwards, C.G., Haag, K.M., Collins, M.D., Hutson, R.A., Huang, Y.C., Lactobacillus kunkeei sp. nov.: a spoilage organism associated with grape juice fermentations, Journal of Applied Microbiology, 1998, 84, 698-702

Sandi, N.A., Salasia, S.I.O., Alternative Antibiotics Source from Symbiont of Lactid Acid Bacteria Inside Stomach of Honeybees (Apis mellifera and Apis dorsata) Against Multiresistant Antibiotics Pathogenic Bacteria, Research Journal of Microbiology, 2016, 11(2-3), 93-100

De Vuyst, L., Leroy, F., Bacteriocins from lactic acid bacteria: Production, purification and food applications, Journal of Molecular Microbiology and Biotechnology, 2007, 13, 194-199

Klaenhammer, T., Altermann, E., Arigoni, F., Bolotin, A., Breidt, F., Broadbent, J., Cano, R., Chaillou, S., Deutscher, J., Gasson, M., van de Guchte, M., Guzzo, J., Hartke, A., Hawkins, T., Hols, P., Hutkins, R., Kleerebezem, M., Kok, J., Kuipers, O., Lubbers, M., Maguin, E., McKay, L., Mills, D., Nauta, A., Overbeek, R., Pel, H., Pridmore, D., Saier, M., van Sinderen, D., Sorokin, A., Steele, J., O'Sullivan, D., de Vos, W., Weimer, B., Zagorec, M., Siezen, R., Discovering lactic acid bacteria by genomics, Antonie van Leeuwenhoek, 2002, 82, 29-58

Estevinho, L., Pereira, A.P., Moreira, L., Dias, L.G., Pereira, E., Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey, Food Chemistry and Toxicology, 2008, 46, 3774-3779


Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Scientific Papers Animal Science and Biotechnologies


Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
_________________________________________________________________

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110