Anti-biofilm and antiradical activity of different essential oils

Miroslava Kačániová


The formation of biofilms is determined by the type of microrganisms, availability of nutrients and the characteristics of substrate. In the present study, we aimed to use matrix-assisted laser desorption ionization time-of-flight mass spectrometry profiling as a methodology to monitor Stenotrophomonas maltophilia biofilm development and evaluate the antibacterial, anti-biofilm and antioxidant activity of Mentha piperita and Pimpinella anisum essential oils against S. maltophilia. Biofilms were grown within polypropylene tubes containing a glass slide and wooden toothpick, and were cultivated 3, 5, 7, 9, 12 or 14 days of inoculation and with essential oils experiment also. Planktonic cells were obtained separately by centrifugation as control. MALDI-TOF experiment were performed, one by collecting biofilms from both the glass slide and the wooden toothpick external surface, and the other by acquiring biofilms from these surfaces after essential oils influence. The molecular results showed that MALDI profiling is able not only to distinguish between different biofilm stages, but it is also appropriate to indicate when the biofilm cells are released at the dispersion stage, which occurred first on glass slide and wooden toothpick. Finally, the present study pointed out that MALDI profiling may emerge as a promising tool for the biofilms formation and control.


antioxidant activity, anti-biofilm activity, mass spectrometry, S maltophilia, essential oils

Full Text:



Bakkali, F., Averbeck, S., Averbeck, D., Idaomar, M., Biological effects of essential oils – a review, Food and Chemical Toxicology, 2008, 46, 446-475

Uniyal, B.P., Sharma, J.R., Choudhery, U., Singh, D.K., Flowering Plants of Uttarakhand (A Checklist), Bishen Singh Mahendra Pal Singh, Deh¬radon, 2007, 404 p.

Gaur, R.D., Flora of the District Garhwal Northwest Himalaya (with Ethnobotanical Notes), Kew Bulletin, 2001, 56(1), 251

Brasil, Farmacopeia Brasileira. Agência Nacional de Vigilância Sanitária, Anvisa, Brasília. 2010, 2, 808 p. 5. Samojlik, I., Mijatović, V., Petković, S., Škrbić, B., Božin, B., The influence of essential oil of aniseed (Pimpinella anisum, L.) on drug effects on the central nervous system, Fitoterapia, 2012, 83, 1466-1473

Özel, A., Anise (Pimpinella anisum): changes in yields and component composition on harvesting at different stages of plant maturity, Experimental Agriculture, 2009, 45, 117-126

Rodrigues, V.M., Rosa, P.T.V., Marques, M.O.M., Petenate, A.J., Meireles, M.A.A., Supercritical extraction of essential oil from aniseed (Pimpi¬nella anisum L) using CO2: solubility, kinetics, and composition data, Journal of Agriculture and Food Chemistry, 2003, 51, 1518–1523.

Ullah, H., Honermeier, B., Fruit yield, essential oil concentration and composition of three anise cultivars (Pimpinella anisum L.) in relation to sowing date, sowing rate and locations, Industrial Crops and Products, 2013, 42, 489-499

Matos, F.J.A., Constituintes químicos ativos e propriedades biológicas de plantas medicinais brasileiras, Editora UFC, Fortaleza, 2004, 448 p.

Nelson, R.R.S., In vitro activities of five essential oils against methicillin-resistant Staphylococcus aureus and vancomycin-reistant Entrococcus faecium, Journal of Antimicrobial Chemotherapy, 1997, 40, 305-306

Nostro, A., Marino, A., Blanco, A.R., Cellini, L., Di Giulio, M., Pizzimenti, F., Roccaro, A.S., Bisignano, G., In vitro activity of Carvacrol against Staphylococcal preformed biofilm by liquid and vapour contact, Journal of Medicinal Microbiology, 2009, 58, 791

Khan M.S.A., Zahin, M., Hasan, S., Husain, F.M., Ahmad, I., Inhibition of quorum sensing regulated bacterial functions by plant essential oils with special reference to clove oil, Letters of Applied Microbiology, 2009, 49, 354-360

Khan, M.S.A., Ahmad, I., In vitro antifungal, anti-elastase and anti-keratinase activity of essential oils of Cinnamomum, Syzygium and Cymbopogon-species against Aspergillus fumigatus and Trichophyton

rubrum, Phytomedicine, 2011, 19, 48-55

Kavanaugh, N.L., Ribbeck, K., Selected antimicrobial essential oils eradicate Pseudomonas spp. and Staphylococcus aureus biofilms, Applied Environmental Microbiology, 2012, 78, 4057-4061

Husain, F.M., Ahmad, I., Asif, M., Tahseen, Q., Influence of clove oil on certain quorum sensing regulated functions and biofilm of Pseudomonas aeruginosa and Aeromonas hydrophila, Journal of Biosciences, 2013, 38, 1-10

Singh, J., Chhikara, B.S., Comparative global epidemiology of HIV infections and status of current progress in treatment, Chemical and Biological Letters, 2014, 1(1), 14-32

Batoni, G., Maisetta, G., Brancatisano, F.L., Esin, S., Campa, M., “Use of antimicrobial peptides against microbial biofilms: Advantages and limits,” Current Medicinal Chemistry, 2011, 18(2), 256-279

Gupta, P., Sarkar, S., Das, B., Bhattacharjee, S., Tribedi, P., “Biofilm, pathogenesis and prevention—a journey to break the wall: a review,” Archives of Microbiology, 2016, 198(1), 1-15

Takaine, M., Imada, K., Numata, O., Nakamura, T., Nakano, K., “The meiosis-specific nuclear passenger protein is required for proper assembly of forespore membrane in fission yeast”, Journal of Cell Science, 2014, 127(20), 4429-4442

Petrovska, B.B., “Historical review of medicinal plants' usage,” Pharmacognosy Reviews, 2012, 6(11), 1-5

Bakkali, F., Averbeck, S., Averbeck, D., Idaomar, M., “Biological effects of essential oils—a review”, Food and Chemical Toxicology, 2008, 46(2), 446-475

Sánchés-Moreno, C., Larrauri, A., Saura-Calixto, F., A procedure to measure the antioxidant afficiency of polyphenols, Journal of Science and Food Agriculture, 1998, 76, 270-276

Ceri, H., Olson, M.E., Morck, D.V., Storey, D.G., Minimal biofilm eradication (MBEC) assay: susceptibility testing for biofilms, In: Pace, J.L., Rupp, M.E., Finch, R.G., editors, Biofilms, infection, and antimicrobial theraphy, CRC Pres, Boca Raton, 2006, pp. 257-269

Adukwu, E.C., Allen, S.C., Phillips, C.A., The anti-biofilm activity of lemongrass (Cymbopogon flexuosus) and grapefruit (Citrus paradisi) essential oils against five strains of Staphylococcus aureus, Journal of Applied Microbiology, 2012, 113(5), 1217-1227

Pereira, F.D.E.S., Bonatto, C.C., Lopes, C.A.P., Pereira, A.L., Silva, L.P., Use of MALDI-TOF mass spectrometry to analyze the molecular profile of Pseudomonas aeruginosa biofilms grown on glass and plastic surfaces, Microbial Pathogenesis, 2015, 86, 32-37

Kačániová, M., Terentjeva, M., Vukovic, N., Puchalski, C., Roychoudhury, S., Kunová, S., Kluga, A., Tokár, M., Kluz, M., Ivanišová, E., The antioxidant and antimicrobial activity of essential oils against Pseudomonas spp. isolated from fish, Saudi Pharmaceutical Journal, 2017, 25(8), 1108-1116

Tutar, U., Çelik, C., Karaman, I., Ataş, M., Hepokur, C., Anti-biofilm and antimicrobial activity of Mentha pulegium L. essential oil against multidrug-resistant Acinetobacter baumannii, Tropical Journal of Pharmaceutical Research, 2016, 15(5), 1039-1046

Bazargani, M.M., Rohloff, J., Anti-biofilm activity of essential oils and plant extracts against Staphylococcus aureus and Escherichia coli biofilms, Food control, 2016, 61, 156-164


  • There are currently no refbacks.

Copyright (c) 2019 Scientific Papers Animal Science and Biotechnologies

Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at]
Phone: +40-256-277160, Fax.: +40-256-277110