Effect of natural carbohydrate fraction from yeast cell wall (NCF) and age on intestinal morphometric parameters of broiler chickens

Mirko Ivkovic, Dragan Žikić, Dejan Beuković, Siniša Bjedov, Lidija Perić

Abstract


Yeast cell wall products are used as feed supplements for increasing animal health and performance. A trial was set up to test effects of natural carbohydrate fraction from yeast cell wall (NCF) on gut parameters that can be correlated with gut health. Trial design included four treatments (control group and three groups with different levels of NCF in feed: 0,02%, 0,04% and 0,08%) and eight replications per treatment. Each replication was one pen with 38 broiler chicks. Chicks were sexed in incubation station and only males were used in the trial. On days 7 and 28 of the trial, after body weight had been measured, chicks with weight aproximate to that of their treatment average were selected for intestinal sampling. Ten chiks per treatment were sacrificed. Central part of jejunum was taken for morphometric analysis and stained with double staining (Alcian Blue and Periodic Acid-Schiff). At least 15 measurements (25 on average) were made per animal for villus height and width, crypt depth and Tunica muscularis thickness. Statistical analysis was performed using factorial ANOVA with feed and age as factors, and with Duncans test. Age significantly affected jejunal morphometry, while effects of NCF were less obvious. Villus height, crypt depth and Tunica muscularis thickness doubled from 7th to 28th day, leaving villus to crypt ratio unchanged. Villus width also increased, but to a lesser extent, making villi of older chicks more elongated. Group receiving 0,02% NCF in feed had smaller crypt depth, and larger villus to crypt ratio, while group receiving 0,08% NCF had smaller villus area (villus height × villus width) compared to the control group.


Keywords


intestinal morphology; mannan-oligosaccharides; broilers

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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
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