Lactic acid bacteria isolated from traditional cheese

Miroslava Kačániová, Soňa Felsöciová, Peter Haščík, Ľudmila Nagyová, Elena Horská, Lucia Godocikova, Ľubomír Lopašovský, Simona Kunová

Abstract


Traditional national cheeses are a source for the search for species and strains of lactic acid bacteria (LAB) which are not within the range of bacterial agents used in the dairy industry. The purpose of our work was isolation and identification of LAB from traditional Slovakian cheeses made from cow's milk. Forty samples of cheese were selected for our research, produced in the four regions of the Slovakia. LAB were cultured on de Man, Rogosa and Sharpe and cultivated for 48 hours at 37 °C in microaerophilic condition. The suspicious colonies from lactic acid bacteria were selected for further confirmation with MALDI-TOF. Selected colonies were cultured overnight on TSA agar anaerobically and used for identification. 166 cultures were isolated from the samples of cheese. Genus and species identification was completed using mass spectrometry method.  Based on the complex of LAB isolated were assigned to the following genera and species: Lactococcus spp., including L.  lactis and L.  garvieae; Lactobacillus spp.: L. curvatus, L. delbrueckii, L. fermentum, L. papacasei, L. paraplantarum, L. plantarum and L. sakei; Enterococcus spp.: E. durans, E. faecalis, E. faecium and E. italicus; Leuconostoc spp.: L. mesenteroides, Pediococcus spp.: P. pentosaceus and Streptococcus spp.: S. salivarius ssp. thermophilus.


Keywords


lactic acid bacteria, MALDI-TOF MS Biotyper, Slovak cheese

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References


Kırmacı, A.H., Özer, B.H., Akçelik, M., Akçelİk, N., Identification and characterisation of lactic acid bacteria isolated from traditional Urfa cheese, International Journal of Dairy Technology, 2016, 69, 301-307

Carr, F.J., Chill, D., Maida, N., The lactic acid bacteria: A literature survey, Critical Reviews in Microbiology, 2002, 28(4), 281-370

Ferreira, C.L.L.F., Grupo de bactérias lácticas caracterização e aplicação tecnológica de bactérias probióticas. In A. Célia L.L.F. Ferreira (Eds.), Prebióticos e Probióticos: Atualização e prospecção, Viçosa, MG: Universidade Federal of Viçosa Press, 2003, 206p.

Fox, P.F., Guinee, T.P., Cogan, T.M., Mcsweeney, P.L.H. Fundamentals of cheese science . Cap. 5. Gaithersburg: Aspen Publishers. 2000, pp. 54-97

Wouters, J.T.M., Ayad, E.H.E., Hugenholtz, J., Smit, G. Microbes from raw milk for fermented dairy products, International Dairy Journal, 2002, 12(2-3), 91-109

Alegría, Á., Álvarez-Martín, P., Sacristán, N., Fernández, E., Delgado, S., Mayo, B. Diversity and evolution of the microbial populations during manufacture and ripening of Casín, a traditional Spanish, starter-free cheese made from cow’s milk, International Journal of Food Microbiology, 2009, 136, 44–51

Ao, X., Zhang, X., Zhang, X., Shi, L., Zhao, K., Yu, J., Dong, L., Cao, Y., Cai, Y., Identification of lactic acid bacteria in traditional fermented yak milk and evaluation of their application in fermented milk products, Journal of Dairy Science, 2012, 95(3), 1073-1084

Giraffa, G., Chanishvili, N., Widyastuti, Y., Importance of lactobacilli in food and feed biotechnology, Research in Microbiology, 2010, 161(6), 480-487

, Kovalenko, N.K., Lashhevskij, V.V. The use of polymerase chain reaction (PCR) for the identification of lactic acid bacteria, Molochna Promislovіst', 2003, 1(4), 24-25

Mayo, B., Caio, T.C., Rachid, C., Alegria, A., Analy, M.O., Leite, R., Peixoto, S., Delgado, S., Impact of Next Generation Sequencing Techniques in Food Microbiology”, Current Genomics, 2014, https://doi.org/10.2174/1389202915666140616233211

Amaral Luana, D.M.F., Silva, F.S., Casarotti, N.L., Sousa Nascimento, C., Penna, A.L.B., Enterococcus faecium and Enterococcus duransisolated from cheese: Survival in the presence of medications under simulated gastrointestinal conditions and adhesion properties, Journal of Dairy Science, 2012, 95(3), 1073-1084

Zhang, F., Jiang, M., Wan, C., Chen, X., Chen, X, Tao, X. Shah, N.P., Wei, H., Screening probiotic strains for safety: Evaluation of virulence and antimicrobial susceptibility of enterococci from healthy Chinese infants, Journal of Dairy Science, 2016, 99(6), 4282-4290

Goh, H.F., Philip, K., Isolation and mode of action of bacteriocin BacC1 produced by nonpathogenic Enterococcus faeciumC1, Journal of Dairy Science, 2015, 98(8), 5080-5090

Zhang, F. Qiu, L., Xu, X., Liu, Z., Zhan, H., Tao, X., Shah, N.P.,Wei, H., Beneficial effects of probiotic cholesterol-lowering strain of Enterococcus faecium WEFA23 from infants on diet-induced metabolic syndrome in rats, Journal of Dairy Science, 2017, 100(3!, 1618-1628

Medeiros, R.S., Araújo, L.M., Queiroga, V., NetoAndrade, P.P., Melo, M.A., Gonçalves, M.M.B.P., Identification of lactic acid bacteria isolated from artisanal Coalho cheese produced in the Brazilian Northeast, CyTA - Journal of Food, 2016, 14(4), 613-620

Terzić‐Vidojević, A., Veljović, K., Tolinački, M., Nikolić, M., Ostojic, M., Topisirović, L., Characterization of lactic acid bacteria isolated from artisanal Zlatar cheeses produced at two different geographical location, Genetika, 2009, 41(1), 117–136

Terzić‐Vidojević, A., Vukasinovic, M.,Veljovic, K., Ostojic, M., Topisirovic, L., Characterization of microflora in homemade semi‐hard white Zlatar cheese, International Journal of Food Microbiology, 2007, 114, 36–42

Abdullah, S.A., Osman, M.M., Isolation and identification of lactic acid bacteria from raw cow milk, white cheese and rob in Sudan, Pakistan Journal of Nutrition, 2010, 9(12), 120–126

Bluma, A., Ciprovica, I., Diversity of lactic acid bacteria in raw milk. In Annual 21st international scientific conference: “Research for rural development”, Jelgava, Latvia, 2015, pp. 157-161

Šaková, N., Sádecká, J., Lejková, J., Puškárová, A., Koreňová, J., Kolek, E., Pangallo, D., Characterization of May bryndza cheese from various regions in Slovakia based on microbiological, molecular and principal volatile odorants examination, Journal of Food and Nutrition Research, 2015, 54(3), 239-251.

Menéndez, S., Centeno, J.A., Godinez, R., Rodriguez‐Otero, J.L., Effects of Lactobacillus strains on the ripening and organoleptic characteristics of Arzúa‐Ulloa cheese, International Journal of Food Microbiology, 2000, 59(1–2), 37-46

Giraffa, G., Functionality of enterococci in dairy products. International Journal of Food Microbiology, 2003, 88(2–3), 215-222.

Strateva, T., Dimov, S.G., Atanasova, D., Petkova, V., Savov, E., Mitov, I., Molecular genetics study of potentially bacteriocinogenic clinical and dairy Enterococcus spp. isolates from Bulgaria, Annals of Microbiology, 2016, 66(1), 381.


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