Ameliorative Effect of Active Principles from Cilantro and Spirulina Powder on Lead Antagonism to Calcium and Magnesium in Carassius gibelio Bloch

Marioara Nicula, Nicolae Pacala, Lavinia Stef, Ioan Pet, Ioan Bencsik, Tiberiu Iancu, Camelia Tulcan, Monica Dragomirescu, Dorel Dronca, Adina Berbecea, Eliza Simiz, Mirela Ahmadi, Adela Marcu, Silvia Erina

Abstract


The aim of this work was to investigate the calcium and magnesium tissue distribution in selected organs of Prussian carp specimens before and after experimental lead intoxication, with and without cilantro and spirulina powder addition in fish diet.

The study involved 120 individuals with similar biometric measurements, caught from a local fish farm and equally divided in four experimental groups, as follows: C group (without treatment), E1 group (75 ppm Pb into water as Pb(NO3)2 x ½H2O), E2 group (75 ppm Pb into water+2% lyophilized cilantro in feed), E3 group (75 ppm Pb into water+2% lyophilized spirulina in feed). Tissue samples (gills, muscle myotomers–epaxial, heart, skin and scales, intestine, liver, brain, gonads, kidney) were collected at the end of a 21 days experimental period.

Atomic absorption measurements of the analytes were carried out with a AAS-VARIAN model.

Data analyses were performed using a SPPS IBM 22 software package. Significance of differences in the means content of calcium and magnesium in fish tissues were statistically analysed by ANOVA completed with post-hoc Tukey’s test.

The calcium and magnesium tissue concentrations were significantly reduced by sublethal lead-induced poisoning (p<0.001). But, coriander and spirulina powder were able to attenuate much of the lead antagonistic effect with respect to calcium absorption and bioavailability, spirulina being more effective in the case of the gill and renal tissue, while coriander in the case of cardiac tissue. Instead, spirulina powder has improved magnesium distribution in all analyzed tissues.

Keywords


calcium; cilantro; fish; lead toxicity; magnesium; natural chelators; spirulina

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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Faculty of Animal Science and Biotechnologies
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
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