Genetic Relationship among Indigenous Goat Populations in Nigeria based on Cellulose Acetate Electrophoresis Systems

Olaoluwa Ganiyu, OSAMEDE HENRY OSAIYUWU, Mabel Omolara Akinyemi, Adebowale Emmanuel Salako


Genetic diversity is the baseline for survivability and improvement of livestock in a wide array of environments. Variations among Red Sokoto, Sahel and WAD goats was determined using 5ml of blood collected by jugular venipuncture from 150 randomly selected goats (50 goats per breed). The samples were subjected to Cellulose Acetate Electrophoresis to determine the genetic variants of Haemoglobin (Hb), Transferrin, Carbonic Anhydrase, Albumin, Esterase 1, Esterase 3 and Alkaline phosphatase. Allele frequencies, similarity indices as well as genetic distances among the breeds were estimated. All the breeds in this study were polymorphic for Haemoglobin, Transferrin, Carbonic Anhydrase, Albumin, Alkaline phosphatase and Esterase 1 except WAD which was monomorphic for Alkaline phosphatase. The frequencies of HbA was considerably higher in Red Sokoto and WAD while frequencies of HbB were higher in Sahel goat population. The significant deviations from HWE (P<0.05, P<0.01 and P<0.001) was observed for some of the loci within the breeds. Genetic distance (D) obtained in this study ranged between 0.0648 and 0.1579 which indicates little genetic differentiation between the breeds. The results of this current study suggest that genetic improvement will be better achieved if WAD and Sahel goats are crossed.


Biochemical characterization, Electrophoresis, Genetic diversity, Hardy-Weinberg Equilibrium, Indigenous goats

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Adebambo, A.O, Adebambo O, Williams J L, Blott S and Urquart B., Genetic distance between two popular Nigerian goat breeds used for milk production. LRRD, 2011. 23 (2).

Adebambo, O.A., Animal genetics and quality of life. Proceedings of the 29th Annual Conference of the Genetics Society of Nigeria, October 11-14, Abeokuta, Nigeria, 2004 pp: 42-53.

Agaviezor, B. O., Ajayi, F. O. & Benneth, H. N., Haemoglobin Polymorphism in Nigerian Indigenous Goats in the Niger Delta Region, Nigeria. International Journal of Science and Nature, 2013 4 (3) 415-419

Akinyemi, M.O. and Salako, A.E., Hemoglobin polymorphism and Morphometrical correlates in the West African Dwarf Sheep of Nigeria. International Journal of Morphology, 2010 28(1) 205-208.

Al-Qarawi, A.A., Ali, B.H. Variations in the Normal Activity of Esterases in Plasma and Liver of Camels (Camelus dromedarius), Cattle (Bos indicus), Sheep (Ovis aries) and Goats (Capra hircus), Journal of Vet Med Series A 2002 50 (4) 201-204

Awobajo, O.K, Salako A.E, Akinyemi M.O and Osaiyuwu O.H., Analysis of genetic structure of West African Dwarf goats by allozyme markers. Small Ruminant Research, 2016 136 145-150

Bindu K.A, Raghavan K.C., Haemoglobin Polymorphism in Malabari Goats. Vet World 2010 3(2) 74-75

Buvanendra, V., Adu, I.F. and Oyejola, B.A., Breed and environmental effects on lamb production in Nigeria. Journal of Animal Science, 1981 96:9-15.

Clarke S.W, Turker E.M, and Osterhoff D.R., Blood groups and biochemical polymorphisms in the Namaqua sheep breed. Anim. Blood Groups Biochem. Genet., 1989 20:279-286.

Das, A.K. and Deb, R., Biochemical polymorphism and its relation with some traits of importance in poultry. Veterinary World, 2008 1, 7, 220-222.

Evans, J.V.H., Warren, L., The distribution of hemoglobin and blood potassium types in British breed of sheep. Proc. Soc. London, 1958 149: 2549-2562.

Guney, O, Ozuyanik, O, Torun, O, Gorgulu, M and Darcan, N., Relationship between some polymorphicparameters and performances in damascus goats. Pak. J. Biol. Sci., 2003 6: 738-740.

Ibeagha-Awemu, E.M., Erhardt, G., An evaluation of genetic diversity indices of the Red Bororo and White Fulani cattle with different molecular markers and their implications for current and future improvement options. Trop. Ani. H. Prod., 2004 38, 431-441.

Kowalska M., Zatoń-Dobrowolska M., Genetic distance between different breeds of sheep. Journal of Agrobiology, 2008 25: 23-26.

Lamorde, A.G., Research priorities for sustainable women empowerment and development. A paper presented at the National conference on research as backbone for sustainable development held at Abuja, Nigeria on 11th-15th Aug. 1997. Cited by Abubakar, M.M. 1998: Osinowo, O.A (Ed). The commerative book: 25 years of the Nigeria Society of Animal Production (NSAP). 1973 -1998.

Legates, J.E. and Warwick, E.J., Breeding and Improvement of Farm Animals. 8th Edition, McGraw-Hill Publishing Co., USA, 1990.

Levene H., On a matching problem in genetics. Ann. Math. Stat., 1949 20: 91-94.

Macaulay, O.O., Effect of milking frequency on secretion rate in Red Sokoto and West African Dwarf goats. In: Animal Agriculture Towards Millennium Development in Nigeria. Proc.33rd Ann. Conf. Nig. Soc. Anim. Prd. (NSAP). Eds. O.A. Adeyemi, A.O. Ogungbesan, A.O. Dada, O.O. Eniolorunda, H.A. Awojobi, D.B. Oke and J.A. Agunbiade, 2008.

Malan, D.D., Scheels, C.W., Buyse, J., Kwakemaak, C. and Siebrits, F. K., Metabolic rate and its relationship with ascites in chicken genotypes. Br. Poult. Sci., 2003 44: 309-315.

Maxwell, C. and Baker, C.M.A., Molecular Biology and the Origin of Species: Heterosis, Protein Polymorphism and Animal Breeding. Sidgwick and Jackson, London, 1980

Miller M.P., TFPGA Version 1.3. A Windows program for the analysis of allozyme and molecular population genetic data. Department of Biological Science. Northern Arizona University, 1997 P 30.

Mujibi, N. F., Genetic characterization of West African Dwarf (WAD) goats using microsatellite markers. MSc thesis submitted to the Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, Kenya, 2005.

Mwacharo. J.M., Otieno, C.J., Okeyo, A.M. and Aman, R.A., Characterization of indigenous fat-tailed and fat-rumped hair sheep in Kenya: Diversity in blood proteins. Trop. Ani. H. Prod, 2002 34, 515-524.

Nei M, Mathematical models of speciation and genetic distance. In: Karlin, S., Nevo, E. (Eds.), Population Genetics and Ecology. (Academic Press, New York), 1976.

Nei M., Genetic distance between populations. American Naturalist, 1972 106, 283-92.

Ngere, L.O., Adu, I.F and Okubanjo, A.O., The Indigenous goats of Nigeria. Ani gen Res Info, 1984 3:1-9.

Okpeku, M., Yakubu, A., Peters, S.O., Ozojie, M.O., Ikeobi, C.O., Adebambo, O.A. and Imumorin, I.G., Application of multivariate principal component analysis to morphological characterization of indigenous goats in Southern Nigeria. Acta agric. Slovenica, 2011 98/2, 101-109.

Osaiyuwu, O.H., Akinyemi, M.O., Omoike, O.A. and Salako, A.E., Heamoglobin polymorphism in Red Sokoto goats of Nigeria. Journal of Animal Production Adv. 2013 3(9) 278-282.

Osinowo, O.A., Livestock sub sectional review. Working paper on small ruminant mimeograph, 1992. Pp 10 – 14.

RIKEN, Genetic Quality Monitoring by Biochemical Isozymes. RIKEN Bioresource Center, 2006.

Rychlik T, Pakulski T and Krawczyk A, Monitoring genetic variation in a conservation flock of Coloured Merino sheep based on blood group and protein polymorphism. Annals of Ani. Sci., 2011 11, 41-52.

Salako, A.E., Ijadunola, T.O. and Agbesola, Y.O., Hemoglobin polymorphism in Nigerian indigenous small ruminant populations - preliminary investigation. Afri J Biotec, 2007 6 (22):2636-2638.

Salako, A.E., Maturity rate of some morphometric traits in the West African Dwarf Sheep of Nigeria. T.Jou Anim Scie 2004 7(1):51-55.

Shahrbabak H.M, Farahani A.H.K, Shahrbabak M.M. and Yeganeh H.M., Genetic variations between indigenous fat-tailed sheep populations. Afr. J. Biotechnol. 2010 9:5993-5996.

Shamsuddin, A. K., Nandakumaran, B. and Mukundan, G., Genetic studies on haemoglobin albumin and amylase polymorphism in Malabari goats and its exotic cross breds. Kerala J. Vet. Sci., 1986 17: 1-6

Takezaki, N. and Nei, M., Genetic distances and reconstruction of phylogenetic tree from microsatellite DNA. Genetics, 1996 144: 389–399.

Wang SQ, Foote WC and Bunch T.D., Transferin and haemoglobin polymorphism in domesticated goats in USA. Anim. Genet., 1991 22(1): 91-94.

WATANABE, S. SUZUKI, S., Studies on Serum Albumin Polymorphism in Goats. Jap. J. Zootech. Sci., 1967 38, 11.

Yeh FC, Yong R., POPGENE version 1.31: Microsoft-based Freeware for Population Genetic Analysis. University of Alberta, Edmonton, Canada, 1999.

Zaragoza, P., Arana, A., Zarazaga, I. and Amorena, B., Blood biochemical polymorphisms in rabbits presently bred in Spain. Genetic variation and distances among populations. Australian Journal of Biological Sciences, 1987 40, 1-12.


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