Effect of ß-lactoglobulin Locus Polymorphism on Milk Related Traits in Romanian Spotted

Radu I. Neamt, Daniela E Ilie, Stelian Acatincai, Isabela Ciabrun, Ludovic Toma Cziszter


The main aim of the study was to assess the influence of ß-lactoglobulin (LGB) genotype on milk related traits in Romanian Spotted (R.S.) breed. Altogether 254 cattle were genotyped for in order to establish the share of A and B allele in LGB locus using PCR-RFLP assay. The most prevalent was A allele (0.662) compared to B allele (0.338). Comparable frequencies (P>0.084) were recorded for AA (0.434) and AB (0.455) genotypes. The BB heterozygous recorded a lower frequency (0.111) compared with others (P≤0.001). For the LGB polymorphism, no significant differences (P>0.05) were observed according to milk production. The higher milk production was associated to AB (6094.31±103.22 kg) compared to AA (5912.22±91.7 kg, P>0.53) and BB (5977.7±81.12 kg, P>0.71) genotypes. The higher fat percentage (4.26±0.02%) was recorded for BB genotype, compared to AA genotype (4.19±0.02%, (P≤0.019). A significantly increased protein percentage was associated with AB genotype (3.43±0.03%) compared with AA (3.28±0.02%, P≤0.027). No significant difference (P>0.66) was recorded compared to BB genotype (3.42±0.01%) related to this trait. The results obtained encourage including marker assisted-selection and use the genotyped sires for genes with economic values in the future breeding scheme of Romanian Spotted breed.


LGB polymorphism; milk related traits; Romanian Spotted

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Aschaffenburg, R., Drewry, J., Occurrence of different betalactoglobulins in cow’s milk, Nature, 1955, 176, 218–219

Caroli, A. M., Chessa, S., Erhard, G. J., Milk protein polymorphisms in cattle: Effect on animal breeding and human nutrition, J. Dairy Sci., 2009, 92, 5335-5352

Ng-Kwai-Hang, K. F., Protein composition of milk and cheesemaking. Modern Dairy, February, 1990, pp14-15

Kim, S., Genetic polymorphism of milk proteins and their association with production traits in Ayrshire, Jersey, Brown and Canadienne., MSc Thesis, 1994, McGill University, Montreal, Canada

Bell, K., One-dimensional starch-gel electrophoresis of bovine skim-milk, Nature, 1962, 195, 705-706

Baker, C. M. A., Manwell, C., Chemical classification of cattle. 1. Breed groups, Anim. Blood Grps. Biochem. Genet., 1980, 11, 127-150

Swaisgood, H., Chemistry of milk proteins. In: Developments in Dairy Chemistry I. Proteins. Ed: P. Fox. Appl. Sci. Publishers, London, 1982, pp. l-59

Aleandri, R., Buttazzoni, G., Schneider, J. C., Caroli, A., Davoli, R., The effects of milk protein polymorphisms on milk components and cheese-producing ability, J. Dairy Sci., 1990, 73, 241

Hill, J. P., The relationship between b-lactoglobulin phenotypes and milk composition in New Zealand dairy cattle, J. Dairy Sci., 1993, 76, 281

Kroeker, E. M., Ng-Kwai-Hang, K. F., Hayes, J. F., Moxley, J. E., Effect of b-lactoglobulin variant and environmental factors on variation in the detailed composition of bovine milk serum proteins, J. Dairy Sci., 1985, 68, 1637

Ng-Kwai-Hang, K. F., Hayes, J. F., Moxley, J. E., Monardes, H. G., Variations in milk protein concentrations associated with genetic polymorphisms and environmental factors, J. Dairy Sci., 1987, 70, 563

Ilie, D. E, Magdin, A., Salajeanu, A., Neamt, R. I., Vintila, I., Influence of CSN3 marker on milk composition in Romanian Brown and Romanian Simmental cattle from SCDCB Arad, Sci. Pap. Anim. Sci. Biotech., 2009, 42, 54-57

Grădinaru, A. C., Ilie, D. E., Creangă, S., The effect of casein genotypes selection on the genetic structure of Romanian Spotted, Holstein Friesian and Montbéliarde cattle populations and the genetic variability of kappa-casein and beta-lactoglobulin in Romanian Grey Steppe, Res. J. Biotech., 2015, 10, 91-98

Grădinaru, A. C., Ilie, D. E., Creangă, S., Milk protein genetic variants in Romanian Spotted, Holstein Friesian and Montbéliarde cows and some correlations with milk parameters, Res. J. Biotech., 2013, 8, 3-9

Walawski, K., Czarnik, U., Zabolewicz, T., Association between beta-lactoglobulin (BLG) polymorphism and indicators of subclinical mastitis in Black-and-White cows, Roczniki Naukowe Zootechniki, 1997, 24, 9-22

Kusza, S., Sziszkosz, N., Nagy, K., Masala, A., Kukovics, S., Andras, J., Preliminary result of a genetic polymorphism of β-lactoglobulin gene and the phylogenetic study of ten urope and central uropean indigenous sheep breeds, Acta Biochim. Pol., 2015, 62, 109-112

Medrano, J. F., Cordova, E. A., Genotyping of bovine kappa-casein loci following DNA sequence amplification, Biotechnology, 1990a, 8, 144-146

Medrano, J. F., Cordova, E. A., Polymerase chain reaction amplification of bovine LGB genomic sequences and identification of genetic variants by RFLP analysis, Anim. Biotech., 1, 1990b, 73-77

Shapiro, S. S., Wilk, M. B., An analysis of variance test for normality (complete samples). Biometrika, 1965, 52, 591–611

Hill, T., Lewicki, P., Statistics: Methods and Applications. StatSoft, 2007, Tulsa, OK, USA

Famula, T. R., Medrano, J. F., Estimation of genotype effects for milk proteins with animal and sire transmitting ability models, J. Dairy Sci., 1994, 77, 3153–3162

Tsiaras, A. M., G. G., Bargouli, G., Banos, C. M., Boscos, Effect of Kappa-Casein and Beta-Lactoglobulin Loci on Milk Production Traits and Reproductive Performance of Holstein Cows, J. Dairy Sci., 2005, 88(1), 327-334

Bonfatti, V., Di Martino, G., Cecchinato, A., Vicario, D., Carnier, P., Effects of β-κ-casein (CSN2-CSN3) haplotypes and β-lactoglobulin (BLG) genotypes on milk production traits and detailed protein composition of individual milk of Simmental cows, J. Dairy Sci., 2010, 93, 3797-3808

Strzalkowska, N., Josef, K., Lech, Z., Zofia, R., Effects of kappa-casein and beta-bactoglobulin loci polymorphism, cows age, stage of lactation and somatic cell count on daily milk yield and milk composition in Polish Black and White cattle, Anim. Sci. Pap. Rep., 2002, 20, 21-35

Ilie, D. E., Sălăjeanu, A., Magdin, A., Stanca C., Vintilă, I., Genetic polymorphism at the β-lactoglobulin locus in a dairy herd of Romanian Spotted and Brown of Maramures breeds, Sci. Pap. Anim. Sci. Biotech., 2008, 41, 104-107

Curi, R. A., Oliveira, H. N., Gimenes, M. A., Silveira, A. C., Lopes, C. R., Effects of CSN3 and LGB gene polymorphisms on production traits in beef cattle. Genetics and Molecular Biology, 2005, 28(2), 262−266

Yahya, M., Ali, A. A., Mohammad, R. N., Ali, E. K., Allelic polymorphism of κ-casein, β-lactoglobulin and leptin genes and their association with milk production traits in Iranian Holstein cattle, J. Cell. Mol. Res., 2013, 5, 75-80

McLean, D. M., Graham, E. R. B., Ponzoni, R. W., Effects of milk protein genetic variants on milk yield and composition, J. Dairy Res., 1984, 51, 531–546

Ojala, M., Famula, T. R., Medrano, J. F., Effects of milk production genotypes on the variation of milk production traits of Holstein and Jersey cows in California, J. Dairy Sci., 1997, 80, 1776–1785

Grădinaru, A. C., Ilie, D. E., Creanga, S., Genetic diversity and phylogenetic relationships based on milk protein genetic variants in Romanian Spotted, Holstein Friesian and Montbéliarde cows, Scientific Papers Animal Science Series, 2014, 61, 35-38

Bovenhuis, H., Van Arendonk, J. A. M., Korver, S., Associations between milk protein polymorphisms and milk production traits, J. Dairy Sci., 1992, 75, 2549–2559

Jairam, B. T., Nair, P. G., Genetic polymorphisms of milk proteins and economic characters in dairy animals, Indian J. Dairy Sci., 1983, 53, 1–8

Meyer, F., Erhardt, G., Failing, K., Senft, B., Relation between milk yield, udder health and milk and blood protein polymorphism in dairy cows, Zuechtungskunde, 1990, 62, 3-14

Matějíček, A., Matějíčková, J., Štípková, M., Hanuš, O., Genčurová, V., Kyseová, J., Němcová, E., Kott, T., Šefrová, J., Krejčová, M., Melčová, S., Hölzelová, I., Bouška, J., Frelich, J., Joint effects of CSN3 and LGB genes on milk quality and coagulation properties in Czech Fleckvieh, Czech J. Anim. Sci., 2008, 53, 246-252

Ahmadi, M., Mohammadi, Y., Darmani, K. H, Osfoori, R., Qanbari, S., Association of milk protein genotypes with production traits and somatic cell count of Holstein Cows, J. Biol. Sci., 2008, 8, 1231-1235

Karim, K., Mohammed, T. B. N., Khalil, M., Amin, A., Hedayatallah, R., Jamal, F., Polymorphism of b-Lactoglobulin gene and its association with milk production traits in Iranian Najdi Cattle, Iranian J. of Biotechnology, 2009, 7, 82-85

Sitkowska, B., Wojciech, N., Agata, M., Slawomir, M., Agnieszka, M., Milk protein polymorphism and effect of herds on cow’s milk composition, Journal of Central European Agriculture, 2013, 14, 78-90

Hill, J. P., The Relationship Between β-Lactoglobulin Phenotypes and Milk Composition in New Zealand Dairy Cattle, J. of Dairy Sci., 1993, 76, 282-286

Dokso, A., Kelava, N., Brka, M., Ivanković A., The effect of LGB genes on quantltative and qualitatlve characteristlcs of milk Holstein breed in Croatia, Proceedings - 22nd International Scientific-Expert Conference of Agriculture and Food Industry – Sarajevo, 2011

Manga, I., Říha, J., Vrtková, I., Polymorphism of CSN3, Pit-1, LGB and its impact on milk performance traits at the Czech Fleckvieh and Holstein breed, Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2008, 56, 131-136

Hristov, P. I., Teofanova, D. R., Mehandzhiyski, I. D., Zagorchev, L. I., Radoslavov, G. A., Significance of Milk Protein Genes Polymorphism for Bulgarian Rhodopean Cattle: Comparative Studies., Biotechnology & Biotechnological Equipment Journal, 2013, 27, 3659-3664

Lum, L. S., Dovc, P., Medrano, J. F., Polymorphisms of Bovine ß-lactoglobulin Promoter and Differences in the Binding Affinity of Activator Protein-2 Transcription Factor, J. Dairy Sci., 1997, 80, 1389–1397

Lunden, A., Nilsson, M., Janson, L., Marked effect of beta-Lactoglobulin polymorphism on the ratio of casein to total protein in milk. J Dairy Sci, 1997, 80, 2996-3005.

Sabour, M. P., Lin, C. Y., Lee, A. J., Mcallister, A. J., Association between milk protein genetics variants bulls for milk yield traits, J. Dairy Sci., 1996, 79, 1050-1056

Kučerová, J., Matějíček, A., Jandurová, O. M., Sørensen, P., Němcová, E., Štípková, M., Kott, T., Bouška, J., Frelich, J., Milk protein genes CSN1S1, CSN2, CSN3, LGB and their relation to genetic values of milk production parameters in Czech Fleckvieh, Czech J. Anim. Sci., 2006, 51, 241-247


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