Identification of IGF-1 gene polymorphism using PCR-RFLP for improving goat meat evaluation in Carpatina breed

Cristina Lazar, Rodica Stefania Pelmus, Alexandru Mihail Gras, Mircea Catalin Rotar, Elena Ghita

Abstract


The objective of this study was to identify insulin growth factor (IGF-1) gene polymorphism using PCR RFLP technique in order to improve goat meat evaluation. Insulin like growth factor -1 play an important stimulator role in skeletal growth, cell differentiation and metabolism. Also it has an important role in control of hair cycles and it is involved in development of wool fiber. Therefore, it is considered as an appropriate candidate gene for meat quality evaluation. Blood samples were collected from 12 Carpatina kids and DNA was extracted using, Wizard Genomic DNA Purification Kit. Polymorphism of IGF-1 was determined by PCR amplification followed by RFLP method using restriction enzyme Hae III. In the present study homozygous individuals were identified by RFLP with enzyme Hae III which cuts the amplicon in two places obtaining genotype BC with three migration bands at 363, 264 and 99 bp. Restriction enzyme doesn’t cut the amplicon of the homozygous individuals for the genotype BB, and there is no restriction site, so amplified DNA fragment migration can be visualized in one band of 363 bp.  Based on results, two genotypes BB and BC were identified. It was identified homozygous genotype BB with 75% and heterozygous genotype BC with 25%. B allele frequency was 0.87 and for allele C was 0.13. Observed and expected values of IGF-1 genotypes were found in Hardy Weinberg equilibrium, after χ2 test was calculated. Further investigation are necessary to be carried out on a high number of animals in order to discover other mutations correlated with meat quality in Carpatina goat.


Keywords


insulin growth factor, goat, meat, evaluation, quality, polymorphism

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References


Balteanu V. A., Vlaic A., Pop F. D., Carsai T. C., Pascal C., Creanga St., Zaharia N., Padeanu I., Voia O. S., Sauer M., Sauer I. V., The study of αS1-casein genetic marker polymorphism in Carpathian goat breed, Scientific paper Animal Husbandry, Iasi, 2010, 53, 321-325

Balteanu I. V. A., Teza de doctorat, Studiul polimorfismelor genetice ale proteinelor majore din lapte la principalele rase de taurine, bubaline, ovine si caprine din Romania in scopul utilizarii lor ca markeri genetici in ameliorare si trasabilitate, 2011, USAMV Cluj Napoca, Facultatea de Zootehnie si Biotehnologii

Lazăr C., Pelmuş R., Marin D., Ghiţă E., Preliminary investigations on milk proteins polymorphism in Carpathian goats and in F1 Carpathian Saanen hybrids, Archiva Zootechnica, 2010, 13:4, 45-57

Miller B. H, Gore A. C., Alterations in hypothalamic insulin-like growth factor-I and its associations with gonadotropin releasing hormone neurons during reproductive development and ageing, 2001 Journal. Neuroendocrinology, 13 (8), 728-736.

Li M., Li Y., Lu L., Wang X., Gong Q., Duan C., Structural, gene expression, and functional analysis of the fugu (Takifugu rubripes) insulin-like growth factor binding protein-4 gene, 2009, American Journal of Physiology Regulatory Integrative Comparative Physiology, 296, R558-R566.

Lan X.Y., Pan C.Y., Chen H., Lei C.Z., Liu S.Q., The Haelll and XspI PCR-RFLPs detecting polymorphisms at the goat IGFBP-3 locus, 2007, Small Ruminant Research, 73, 283-286.

Zhang C, Zhang W, Luo H, Yue W, Gao M, Jia Z (2008). A new single nucleotide polymorphism in the IGF-I gene and its association with growth traits in the Nanjiang Huang goat. Asian Austral Journal of Animal Science, 21, 1073-1079.

Lan X.Y., Pan C.Y., Chen H., Lei C.Z., A DdeI PCR-RFLP detecting genetic variation of goat POU1F1 gene, 2007, Canadian Journal Animal Science, 87, (1), 13-14.

Kumar P., Choudhary V., Kumar K.G., Bhattacharya T.K., Bhushan B., Sharma A., Mishra A., Nucleotide sequencing and DNA polymorphism studies on IGFBP-3 gene in sheep and its comparison with cattle and buffalo, 2006, Small Ruminant Research, 4, 285-292.

Lok F., Owens J. A., Mundy L., Robinson J. S., Owens P. C., Insulin-like growth factor 1 promotes growth selectively in fetal sheep in late gestation, 1996, American Journal of Physiology Regulatory Integrative Comparative Physiology, 270, 1148-1155.

Jensen, E. C., Harding J. E., Bauer M. K, Gluckman P. D., Metabolic effects of IGF-I in the growth retarded fetal sheep, 1999, Journal of Endocrinology, 161, 485-494.

Yakar, S., Rosen C. J., Beamer W. G., Ackert-Bicknell C. L., Wu Y., Liu J. L., Guck T. O., Setser T.O., Frystyk J., Boisclair Y. R., Leroith D., Circulating levels of IGF-1 directly regulate bone growth and density, 2002, Journal of Clinical Investigation, 110, 771-781.

Deng C., Ma R., Yue X., Lan X., Chen H., Lei C, Association of IGF-I gene polymorphisms with milk yield and body size in Chinese dairy goats, 2010, Genetic Molecular Biology, 33, 266-270.

Qiong, W., Fang C., Liu W. J., Fang Y., Gang Y. S., A novel mutation at exon 4 of IGF-1 gene in three indigenous goat breeds in China, 2011, Asian Journal of Animal Veterinary Advances, 6, (6), 627-635.

Sharma A., Dutt G., Jayakumar S., Saroha V., Singh M. K., Pathodiya O. P., Khaddae B. S., Dixit S. P., Novel SNPs in IGF1, GHR and IGFBP-3 genes reveal significant association with growth traits in Indian goat breeds, 2013, Small Ruminant Research, 115, 7-14.

Chung E. R., Kim W. T., Association of SNP marker in IGF-1 and MYF5 candidate genes with growth traits in Korean cattle, 2005, Asian Australas Journal of Animal Science, 18, 1061-1065.

Bennett A. K., Hester P. Y., Spurlock D. E., Polymorphisms in vitamin D receptor, osteopontin, insulin-like growth factor 1 and insulin and their association with bone, egg and growth traits in a layer-broiler cross in chickens, 2006, Animal Genetics, 37, 283-286.

Estany J., Tor M., Villalba D., Bosch L., Gallardo D., Jimenez N., Altet L., Noguera J. L., Reixach J., Amills M., Sanchez A., Association of CA repeat polymorphism at intron 1 of insulin-like growth factor (IGF-I) gene with circulating IGF-I concentration, growth, and fatness in swine, 2007,. Physiology Genomics, 31, 236-243.

Niu, P., Kim S. W., Choi B. H., Kim T. H., Kim J. J., Kim K. S., Porcine insulin-like growth factor 1 (IGF1) gene polymorphisms are associated with body size variation, 2013, Genes Genomics, 35, 523-528.

Amills, M., Jimenez N., Villalba D., Tor M., Molina E., Cubilo D., Marcos C., Francesch A., Sanchez A., Estany J., Identification of three single nucleotide polymorphisms in the chicken insulin-like growth factor 1 and 2 genes and their associations with growth and feeding traits, 2003, Poultry Science, 82, 1485-1493.

Wang W. J., Ouyang K., Ouyang J., Li H. H., Lin S. M., Sun H., Polymorphism of insulin-like growth factor I gene in six chicken breeds and its relationship with growth traits, 2004, Asian Australs Journal of Animal Science, 17, 301-304.

Alakilli S. Y. M., Mahrous K. F., Salem L.M., Ahmed E.S., Genetic polymorphism of five genes associated with growth traits in goat, 2012, African Journal of Biotechnology, 11, (82), 14738-14748

Liu W. J., Fang G.X., Fang Y., Shigang Y., The polymorphism of IGF-1 Gene on tow Goat Breeds in China, Journal of Animal and Veterrinary Advances, 2010, 9, (4), 790-794.


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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

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