Identification of lactic acid bacteria in milk and milk products with MALDI-TOF mass spectrometry

Miroslava Kačániová, Margarita Terentjeva, Lucia Godočíková, Czeslaw Puchalski, Simona Kunová, Maciej Kluz, Rafal Kordiaka, Peter Haščík

Abstract


Recently, the Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has been developed as vigorous method for the identification of microorganisms. In this study, the culturable population of lactic acid bacteria (LAB) from nonpasteurized milk and milk products was identified using the MALDI-TOF MS. Altogether, 20 samples of Slovak milk and milk products were tested, including 10 samples of milk and 10 samples of dairy products. The LAB were cultured on MRS agar at 30 °C. Gram-positive and catalase-negative strains were subjected to identification by the MALDI-TOF MS profiling. MALDI-TOF MS identification allowed the identification of three genera belonging to the LAB -  Lactobacillus, Lactococcus and Leuconostoc. Presence of Lactobacillus was found in 17, Leuconostoc in three and  Lactococcus only in one sample. Lactobacillus spp. was the most abundant genus and comprised 84% of all strains were isolated. Lactobacillus was mostly represented with four species:  Lactobacillus plantarumL. acidophilus, L. ultunensis, L. casei. Our study shows that the MALDI-TOF MS could serve an important method in identidication of microorganisms for dairy industry needs.


Keywords


MALDI TOF MS Biotyper, Lactobacillus, milk and milk products

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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Faculty of Animal Science and Biotechnologies
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
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