The effect of the humic substances, garlic (Allium sativum L.), wormwood (Artemisia absinthium) and walnut (Juglans regia) on growth parameters and mortality of broiler chickens

Veronika Pistová, Henrieta Arpášová, Cyril Hrnčár


The aim of this experiment was to determine the influence of humic substances, garlic (Allium sativum L.), wormwood (Artemisia absinthium) and walnut (Juglans regia) on growth performance and mortality of broiler chickens. A total of 60 Ross 308 broiler chickens were divided into 3 groups (n=20). The control group of chickens was fed with complete feed mixtures without any additives. Chickens in the first experimental group E1 were fed a diet containing 1.5% of humic substances, 0.4% of garlic powder and 0.1% of wormwood. Chickens in the second experimental group E2 were fed a diet containing 1.5% of humic substances, 0.4% of garlic powder and 0.1% of walnut. The body weight, feed intake, feed conversion and total mortality were recorded in 42 day of fattening. At the end of the experiment was average body weight significantly higher (P≤0.01) in the first experimental group compared to the control group (values in the order of the groups: 1801.04±166.25; 1928.26±226.37 and 1850.24 g±SD). The feed intake was in the control group 3.59 kg per chicken, in the first experimental group E1 3.58 kg per chicken and in the second experimental group E2 3.45 kg per chicken. Feed conversion was in control group 1.95 kg, in the first experimental group E1 1.87 kg and in the second experimental group E2 1.84 kg with no significant different (P≥0.05) compared to the control group. Total mortality in 42 day was positive affected in the second experimental group, but with no significant different (P≥0.05) compared to the control group.


broiler chickens, humic substances, garlic, wormwood, walnut, growth, mortality.

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Peña-Méndez E. M., Havel J., Patočka J. Humic substances-compounds of still unknown structure: Applications in agriculture, industry, environment, and biomedicine. Journal of Applied Biomedicine, 3, 2005, 13-24.

Nagaraju, R., Reddy, B.S., Gloridoss, R., Suresh, B.N., Ramesh, C. Effect of dietary supplementation of humic acids on performance of broilers. Indian Journal of Animal Sciences, 84, 2014, 447–452.

Rath, N.C., Huff, W.E., Huff, G.R., Effects of humic acid on broiler chickens, Poultry Science, 2006, 85, 410-414.

Puvaca, N., Kostadinovic, Lj., Popovic, S., et al. Proximate composition, cholesterol concentration and lipid oxidation of meat from chickens fed dietary spice addition (Allium sativum, Piper nigrum, Capsicum annuum). Animal Production Science, 56, 2016, 1920-1927.

Mansoub, N.H. Comparative Effects of Using Garlic as Probiotic on Performance and Serum Composition of Broiler Chickens. Annals of Biological Research, 2, 2011, 486-490.

Fiamegos, Y.C., Kastritis, P.L., Exarchouv, V., et al. Antimicrobial and efflux pump inhibitory activity of caffeoylquinic acids from Artemisia absinthium against gram-positive pathogenic bacteria. Plos One 6, 2011, 1-12.

Kocabağli, N., Alp, M. The Effect of Dietary Humate Supplementation on Broiler Growth and Carcass Yield. Poultry Science, 81, 2002, 227-230.

Karaoglu, M., Macit, M., Esenbuga, N., et al. Effect of Supplemental Humate at Different Levels on the Growth Performance, Slaughter and Carcass Traits of Broilers. Poultry Science, 3, 2004, 406-410.

Kirubakaran, A., Moorthy, M., Chitra, R., et al. Influence of combinations of fenugreek, garlic, and black pepper powder on production traits of the broilers. Veterinary World, 9, 2016, 470-474

Kostadinovic, L., Levic, J., Popovic, S., et al. Dietary inclusion of Artemisia absinthium for management of growth performance, antioxidative status and quality of chicken meat. European Poultry Science, 79, 2015.

Rahamatnejad, E., Roshanfekr, O., Ashayerizadeh, M., Mamooee, M., Ashayerizadeh, A. Evaluation of several non-antibiotics additives on growth performance of broiler chickens. Journal of Aminal and Veterinary Advances, 8, 2009, 1670-1673.

Ramiah, S. K., Zulkifli, I., Rahim, N. A. A. et al. Effects of Two Herbal Extracts and Virginiamycin Supplementation on Growth Performance, Intestinal Microflora Population and Fatty Acid Composition in Broiler Chickens. Asian-Australasian Journal of Animal Sciences, 27, 2014, 375-382.

Javandel, F., Navidshad B., Seifdavati J., Pourrahimi G.H., Baniyaghoub S. The favorite dosage of garlic meal as a feed additive in broiler chickens ratios. Pakistan Journal of Biological Sciences, 11, 2008, 1746-9.

Edmonds, M. S., Johal, S., Moreland, S. Effect of supplemental humic and butyric acid on performance and mortality in broilers raised under various environmental conditions. Journal Of Applied Poultry Research, 23, 2014, 260-267.

Brzoska, F., Sliwinski, B., Michalik-Rutkowska, O., et al. The Effect of Garlic (Allium Sativum L.) on Growth Performance, Mortality Rate, Meat and Blood Parameters in Broilers. Annals of Animal Science, 15, 2015, 961-975.


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