Studies on regulation of the cell cycle in fission yeast.

Miroslava Požgajová, Peter Androvič, Anna Trakovická

Abstract


All living organisms including plants and animals are composed of millions of cells. These cells perform different functions for the organism although they possess the same chromosomes and carry the same genetic information. Thus, to be able to understand multicellular organism we need to understand the life cycle of individual cells from which the organism comprises. The cell cycle is the life cycle of a single cell in the plant or animal body. It involves series of events in which components of the cell doubles and afterwards equally segregate into daughter cells. Such process ensures growth of the organism, and specialized reductional cell division which leads to production of gamets, assures sexual reproduction. Cell cycle is divided in the G1, S, G2 and M phase. Two gap-phases (G1 and G2) separate S phase (or synthesis) and M phase which stays either for mitosis or meiosis. Essential for normal life progression and reproduction is correct chromosome segregation during mitosis and meiosis. Defects in the division program lead to aneuploidy, which in turn leads to birth defects, miscarriages or cancer.

Even thou, researchers invented much about the regulation of the cell cycle, there is still long way to understand the complexity of the regulatory machineries that ensure proper segregation of chromosomes. In this paper we would like to describe techniques and materials we use for our studies on chromosome segregation in the model organism Schizosaccharomyces pombe.

Keywords


meiosis; mitosis; live cell imaging; Schizosaccharomycesn pombe

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References


CIPAK, L., ZHANG, C., KOVACIKOVA, I., RUMPF, C., MIADOKOVA E., SHOKAT, KM., et al. Generation of a set of conditional analog-sensitive alleles of essential protein kinases in the fission yeast Schizosaccharomyces pombe. Cell Cycle, 2011, 3527-3532

DING, D.Q., HARAGUCHI, T., HIRAOKA, Y. From meiosis to postmeiotic events: Alignment and recognition of homologous chromosomes in meiosis. FEBS Journal, 2010, 565-570

DUDAS, A., AHMAD, S., GREGAN, J. Sgo1 is required for cosegregation of sister chromatids during achiasmate meiosis I. Cell Cycle, 2011, 951-955

FORSBURG, S. L. The best yeast? Trends Genet, 1999, 340-344

GAO, Q., TANAKA, T.U., HE, X. GFP-based Microscopic Approaches for Whole Chromosome Analysis in Yeast. In STANSFIELD, I. and STARK, M.J.R. Yeast Gene Analysis. London: Academic Press, 2011, 730

GREGAN, J., RIEDEL, C. G., PIDOUX, A. L., KATOU, Y., RUMPF, C., SCHLEIFFER, A., et al. The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation. Curr Biol., 2007, 1190-11200

HASSOLD, T., HALL, H., HUNT, P. The origin of human aneuploidy: where we have been, where we are going. Human Molecular Genetics, 2001, 203-208

HARIGAYA, Y., YAMAMOTO, M. Molecular mechanisms underlying the mitosis-meiosis decision. Chromosome Research, 2007, 523-537

KOVÁČIKOVÁ, I. et al. A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe. Cell Cycle, 2013, 618-624

LELAND, B.A., KING, M.C. Using LacO arrays to monitor DNA double-strand break dynamics in live Schizosaccharomyces pombe cells. Methods Mol. Biol., 2014, 127-141.

MICHAELIS, C., CIOSK, R., NASMYTH, K. Cohesins: chromosomal proteins that prevent premature separation of sister chromatids. Cell, 1997, 35-45

NURSE, P., BISSETT, Y. Gene required in G1 for commitment to cell cycle and in G2 for control of mitosis in fission yeast. Nature, 1981, 558-560

PIDOUX, A.L., UZAWA, S., PERRY, P.E., CANDE, W.Z., AND ALLSHIRE, R.C. Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast. J. Cell Sci., 2000, 4177–4191

RABITSCH, K. P. et al. Two Fission Yeast Homologs of Drosophila Mei-S332 Are Required for Chromosome Segregation during Meiosis I and II. Current Biology, 2004, 287-301

RUMPF, C. et al. Casein kinase 1 is required for efficient removal of Rec8 during meiosis I. Cell Cycle, 2010, 2657–2662

RUMPF, C., CIPAK, L., SCHLEIFFER, A., PIDOUX, A., MECHTLER, K., TOLIC-NORRELYKKE, I. M., et al. Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4. Cell Cycle, 2010, 3997-4004

SABATINOS, S.A., FORSBURG, S.L. Molecular genetics of Schizosaccharomyces pombe. Methods Enzymol., 2010, 759-795

SEN, S. Aneuploidy and cancer. Curr. Opin. Oncol., 2000, 82-88

WOOD, V., GWILLIAM, R., RAJANDREAM, M. A., LYNE, M., LYNE, R., STEWART, A., et al. The genome sequence of Schizosaccharomyces pombe. Nature, 2002, 871-880


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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
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ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
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FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
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