The effect of probiotics on broiler growth and intestinal morphology when used to prevent Campylobacter jejuni colonization

Lavinia Ştef, Gabi Dumitrescu, Eliza Simiz, Ada Cean, Calin Julean, Ducu Sandu Stef, Elena Pet, Ioan Pet, Voichita Gherasim, Nicolae Corcionivoschi


The aim of this work was to establish the effect of probiotic microorganisms on growth performance and intestinal changes caused by Campylobacter jejuni colonization.In this respect, we used four probiotic microorganisms, namely: Lactobacillus paracasei JR, L. rhamnosus 15b, Y L. lactis and L. lactis FOA.The administration of probiotic microorganisms in different combinations and in different periods of growth does not significantly influence the bioproductive indices of broilers,that is,the total gain, feed intake and FCR (p>0.05). After studying the intestinal mucosa, it was concluded that the four microorganisms administered in broilers’s food determineschanges in the mucosa, inhibiting the development of Campylobacter jejuni,by the presence of smaller caliciform cells and the presence ofreduced leukocyte infiltration in the chorion of the mucosal.


probiotics, pathogenic microorganisms, broiler, intestinal mucosa, bioproductive performances

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Cox. N. A., Cason. J. A., Richardson. L. J., Minimization of Salmonella contamination on raw poultry, Annu Rev Food Sci Technol.;2:75-95. doi: 10.1146/annurev-food-022510-133715. Review, (2011);

Zhao. C., Villena. B. GE. J. De., Sudler. R., Yeh. E., Zhao. S., White. D. G., Wagner. D., Meng .J., Prevalence of Campylobacter spp., Escherichia coli, and Salmonellaserovars in retail chicken, turkey, pork, and beef from the greater Washington, DC, area. Appl. Environ.Microbiol.67:5431 5436.doi:10.1128/AEM.67.12.5431-5436, (2001);

Dibner.J.J., and Richards.J.D., Antibiotic growth promoters in agriculture: history and mode of action. Poult. Sci. 84:634-643,(2005);

Reid.G., Jass.J., Sebulsky., M.T., Mc.Cormick. J.K., Potential uses of probiotics in clinical practice. Clin. Microbiol. Rev.16:658-672,(2003).

Higgins.S. E., J. P., Wolfenden. A. D., Higgins. S., Henderson. N., Torres-Rodriguez. A., Tellez. G., and Hargis. B.,Evaluation of a Lactobacillus-Based Probiotic Culture for the Reduction of SalmonellaEnteritidis in Neonatal Broiler Chicks,Poultry Science 87(1):27-31doi:10.3382/ ps.2007-00210, (2008);

Fallah. R., Saghafi. M., Rezaei. H., and Parvar .R., Effect of Bioplus 2B® and Protoxin Probiotics Supplementation on Growth Performance, Small Intestinal Morphology and Carcass Characteristics of Broiler Chickens, British Journal of Poultry Sciences 2 (2): 11-15, DOI: 10.5829/idosi.bjps.2013.2.2.73137 (2013); jh

Liu.X., YAN.H., Lv. L., Xu. Q., Yin. C., Zhang. K., Wang. P., and Hu. J.,Growth Performance and Meat Quality of Broiler Chickens Supplemented with Bacillus licheniformis in Drinking Water Asian-Australas J Anim Sci. May 2012; 25(5): 682–689. Published online May 1,. doi: 10.5713/ajas.2011.11334, (2012);

Langout .P., New additives for broiler chickens. Feed Mix. pp: 24-27, (2000);

Boostani. A., Mahmoodian. Fard., Ashayerizadeh. H R.. A., & Aminafshar. M., Growth performance, carcass yield and intestinal microflora populations of broilers fed diets containing thepax and yogurt. Revista Brasileira de Ciência Avícola, 15(1), 1-6,(2013);

Panda. K. A., Raju. M.V.L.N., Rama Rao S.V., Sharma S.R, The influence of supplementation of Lactobacillus sporogenes on the performance of broilers, Indian Journal of Animal Nutrition:, Volume: 22, Issue: 1pp 37 40,(2005);

Kim. Y. S., and Ho. S. B., Intestinal Goblet Cells and Mucins in Health and Disease:Recent Insights and Progress, Curr Gastroenterol Rep, 12:319–330: doi 10.1007/s11894-010-0131-2,(2010);

Lievin .V., - Moal. Le., Servin. AL., The front line of enteric host defense against unwelcome intrusion of harmful microorganisms: mucins, antimicrobial peptides, and microbiota. Clin Microbiol, 19:315–337Rev (2006);

Dharmani.P., Srivastava.V., Kissoon-Singh.V., Role of intestinal mucins in innate host defense mechanisms against pathogens. J Innate Immun,1:123–135,(2009);

Neutra. M. R. and Schaeffer. S. F., Membrane interactions between adjacent mucous secretion granules. J. Cell Biol., 74:983-991, (1977);

McCool. D. J., Forstner. J. F., and Forstner. G. G., Synthesis and secretion of mucin by the human colonic tumour cell line LS180, Biochem. J. 302, 111-118, (1994).


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