In vitro antimicrobial activity of four Slovak medicinal plants against different strains of bacteria

Miroslava Kačániová, Jana Petrová, Attila Kántor, Margarita Terentjeva, Maciej Kluz

Abstract


Medicinal plants are traditionally used for the treatment in human medicine.  The antibacterial activity of ethanol extract of four plant species (Achillea millefolium L., Agrimonia eupatoria, Melissa officinalis and Tilia platyphyllos) applied in the traditional medicine in Slovakia were tested. Extracts of certain parts of these plants were tested in vitro against six bacterial species (Bacillus cereus, Enterococcus faecalis, Escherichia coli, Lactobacillus brevis, Lactobacillus hilgardii and Serratia rubidea) strains using the disc diffusion method and microbroth dilution method. Minimum inhibitory concentrations for the extracts against all the microorganisms were determined by serial dilutions. All the extracts demonstrated antibacterial activity against Gram-positive bacteria and partially against Gram-negative bacteria.


Keywords


Antimicrobial activity. Traditional medicinal plants. Minimum inhibitory concentration (MIC). Ethanolic extract. Disc diffusion method.

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References


Singh, M., Singh, N., Khare, P.B., Rawat, A.K.S., Antimicrobial activity of some important Adiantum species used traditionally in indigenous systems of medicine, Journal of Ethnopharmacology, 2008,115, 327–329

Garza, B.A.A., Gonzalez, G.M.G., Aranda, R.S., Torres, N.W., Galindo, V.M.R., Screening of antifungal activity of plants from the northeast of Mexico, Journal of Ethnopharmacology, 2007, 114, 468–471

Kuete, V., Nguemeving, J.R., Beng, V.P., Azebaze A.G..B., Etoa, F.X.. Meyer, M., Bodo, B., Nkengfack, A.E., Antimicrobial activity of the methanolic extracts and compounds from Vismia laurentii De Wild (Guttiferae), Journal of Ethnopharmacology, 2007, 109, 372–379

Kotzekidou, P., Giannakidis, P., Boulamatsis, A., Antimicrobial activity of some plant extracts and essential oils against food borne pathogens in vitro and on the fate of inoculated pathogens in chocolate, Food of Science Technology, 2008, 41, 119–127

Baretta, I. P., Felizardo, R. A., Bimbato, V. F., Santos, M. G. J., Kassuya, C. A. L., Junior,G., et al. Anxiolytic-like effects of acute and chronic treatment with Achillea millefolium L. extract, Journal of Ethnopharmacology, 2012, 140, 46–54

Candan, F., Unlu, M., Tepe, B., Daferera, D., Polissiou, M., Sokmenc, A., et al., Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp. millefolium Afan. (Asteraceae), Journal of Ethnopharmacology, 2010, 87, 215–220

Carvalho, A. M., Plantas y sabiduria popular del Parque Natural deMontesinho. Un estudioetnobotanico en Portugal. Biblioteca de Ciencias.Consejo Superior de Investigaciones Cientificas, Madrid, Spain, 2010, 35

Cavalcanti, A. M., Baggio, C. H., Freitas, C. S., Rieck, L., Sousa, R. S., Santos, J. E. S., et al. Safety and antiulcer efficacy studies of Achillea millefolium L. after chronic treatment in Wistar rat, Journal of Ethnopharmacology, 2006, 107, 277–284

Dall’Acquaa, S., Bolegob, C., Cignarellab, A., Gaionb, R. M., Innocentia, G.. Vasoprotective activity of standardized Achillea millefolium extract, Phytomedicine, 2011, 18, 1031–1036

Jonsdottir G., Omarsdottird S., Vikingssona A., Hardardottirc I., Freysdottir J., Aqueous extracts from Menyanthes trifoliate and Achillea millefolium affect maturation of human dendritic cells and their activation of allogeneic CD4+ T cells in vitro, Journal of Ethnopharmacology, 2011, 136, 88–93

Potrich, F.B., Allemand, A., Silva, L.M., Santos, A.C., Baggio, C.H., Freitas, C.S. et al., Antiulcerogenic activity of hydroalcoholic extract of Achillea millefolium L.: Involvement of the antioxidant system,

Journal of Ethnopharmacology, 2010, 130, 85–92

Trumbeckaite, S., Benetis, R., Bumblauskiene, L., Burdulis, D., Janulis, V., Toleikis, A., et al., Achillea millefolium L. s.l. herb extract: Antioxidant activity and effect on the rat heart mitochondrial functions, Food Chemistry, 2011, 127, 1540–1548

Duke, J.A., Handbook of Medicinal Herbs, 2nd ed. CRC PressLLC, USA, 2002, pp. 4–5

Horikawa, K., Mohri, T., Tanaka, Y., Tokiwa, H., Moderateinhibition of mutagenicity and carcinogenicity of benzo[a]pyrene, 1,6-dinitropyrene and 3, 9-dinitrofluoranthene by Chinese medicinal herbs. Mutagenesis, 1994, 9, 523–526.

Park, E.J., Oh, H., Kang, T.H., Sohn, D.H., Kim, Y.C., Anisocoumarin with hepatoprotective activity in Hep G2 and primary hepatocytes from Agrimonia pilosa, Archive of Pharmacology Research,2004, 27, 944–946

Kwon, D.H., Kwon, H.Y., Kim, H.J., Chang, E.J., Kim, M.B., Yoon,S.K., Song, E.Y., Yoon, D.Y., Lee, Y.H., Choi, I.S., Choi, Y.K., Inhibition of hepatitis B virus by an aqueous extract of Agrimonia eupatoria L., Phytotherapy Research, 2005, 19, 355–358

Bae, J.H., Sohn, M.A., Effect of Agrimonia pilosa L. extract on the growth of food-borne pathogens, Korean Journal of Nutrition, 2005, 38, 112–116

Correia, H.S., Batista, M.T., Dinis, T.C., The activity of anextract and fraction of Agrimonia eupatoria L. against reactive species, Biofactors, 2007, 29, 91–104

Encalada, A.M., Hoyos, M.K., Rehecho, S., Berasategi, I., Garcia-Iñiguez de Ciriano, M., Ansorena, D., et al. Anti-proliferative effect of Melissa officinalis on human colon cancer cell line, Plant Food and Human Nutrition, 2011, 66(4), 328–334

Fazli, D., Malekirad, A., Pilevarian, A., Salehi, H., Zerratpishe, A., Akbar Male, A., et al., Effects of Melissa officinalis L. on oxidative status and biochemical parameters in occupationally exposed workers to aluminum: A before after clinical trial, International Journal of Pharmacology, 2012, 8(5), 455–458

López, V., Martin, S., Gomez-Serranillos, M.P., Carretero, M.E., Jager, A.K., Calvo, M.I., Neuroprotective and neurological properties of Melissa officinalis, Neurochem Research, 2009, 34(11), 1955–1961

Jun, H., Lee, J.H., Jia, Y., Hoang, M., Byun, H., Kim, K.H., et al., Melissa officinalis essential oil reduces plasma triglycerides in human apolipoprotein E2 transgenic mice by inhibiting sterol regulatory element-binding protein-1c-dependent fatty acid synthesis, Journal of Nutrition, 2012, 142(3), 432–440

Taiwo, A.E., Leite, F.B., Lucena, G.M., Barros, M., Silveira, D., Silva, M.V., et al., Anxiolytic and antidepressant-like effects of Melissa officinalis (lemon balm) extract in rats: Influence of administration and gender, Indian Journal of Pharmacology, 2012, 44(2), 189–192

Clinical and Laboratory Standard Institute. Performance standard for antimicrobial susceptibility testing. Wayne, PA: Clinical and Laboratory Standard Institute; 2009. [Online] Available from: http://www.techstreet.com/products/1760826. [Accessed on 22nd January, 2014].

Essawi, T., Srour, M., Screening of some Palestinian medicinal plants for antibacterial activity, Journal of Ethnopharmacology, 2000, 70, 343–349.

Mazandarani, M., Mirdeilami, S.Z., Pessarakli, M., Essential oil composition and antibacterial activity of Achillea millefolium L. from different regions in Northeast of Iran, Journal of Medicinal Plants Research, 2013, 7(16), 1063-1069

Ghaima, K.K., Antibacterial and wound healing activity of some Agrimonia eupatoria extracts, Journal of Baghdad for Sciences, 2013, 10(1), 152-160

Romeo, V., Serena De Luca Piscopo, A., Poiana, M., Antimicrobial effect of some essential oils, Journal of Essential Oils and Research, 2008, 20, 373-379

Hussain, A.I., Anwar, F., Nigam, P.S., Saker, S.D., Moore, J.E., Rao, J.R. et al., Antimicrobial activity of some Lamiaceae essential oils using resazurin as an indicator of cell growth. LWT- Food Science and Technology, 2011, 44, 1199-206

Ahmadian-Attari, M.M., Monsef Esfahani, H.R., Amin, G.R,. Fazeli, M.R. The ethnopharmacological study on antibacterial activity of some selected plants used in Iranian traditional medicine, Journal of Medicinal Plant, 2009, 8(31), 50-57

Dukic, N.M., Bozin, B., Sokovic, M., Simin, N., Antimicrobialand antioxidant activities of (Lamiaceae) essential oil, Journal of Agriculture and Food Chemistry, 2004, 52, 2485-2489

Mencherini, T., Picerno, P., Scesa, C., Aquino, R., Triterpene, antioxidant and antimicrobial compounds from Melissa officinalis, Journal of Natural Products, 2007, 70, 1889-94

Romeo, V., Serena De Luca Piscopo, A., Poiana, M., Antimicrobial effect of some essential oils, Journal of Essential Oil Research, 2008, 20, 373-379

Tullio, V., Nostro, A., Mandras, N., Dugo, P., Banche, G.,Cannatelli, M.A. et al. Antifungal activity of essential oils against filamentous fungi determined by broth microdilution, Journal of Applied Microbiology, 2007, 102, 1544-50


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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

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