HISTOLOGICAL STRUCTURE OF MUSCULUS LONGISSIMUS DORSI IN PIGS WITH THE SAME RYANODINE RECEPTOR GENOTYPE (CC)IN RELATION TO FEEDING INDICATORS

S. HLUCHÝ, Z. ELIÁŠ, R. TOMAN

Abstract


The aim of this study was to investigate the histological structure of pig longissimus muscle in relation to feeding indicators. A total number of 16 pigs about 101.28 kg average live weight with CC RYR -1 genotype were used. Animals were raised on Fattening and Carcass Value Experimental Station (FCVES) of SUA in Nitra on equal conditions, fed with standard nutrition fortified with vitamin-mineral mixture and slaughtered on experimental abattoir of FCVES. Samples from musculus longissimus dorsi (MLD) for histological evaluation were taken within 30 minutes after slaughter, immediately frozen in liquid nitrogen and stored at temperature -20 °C. On FCVES feeding indicators were observed. Samples were histochemically processed and single types of muscle fibbers were differentiated according to reaction on SDH on the basis of method by Vacek (1974). Microscopic system Nikon, digital camera Pixelink and software for image analyse LUCIA for morphometric analysis of MLD structure were used. Histological analysis of MLD showed, highest abundance of white and lowest abundance of intermediate muscle fibbers. Concerning to connective and fat tissue the highest values of connective tissue abundance were obtained. In relation to the average muscle fibber diameter in longissimus muscle, the highest values of white muscle fibber diameter and the lowest values of red muscle fibre diameter were obtained. Correlation analysis of MLD structure and feeding indicators showed significant negative correlations between intermediate fibber abundance and live weight at birth. Concerning to the daily gain it was shown positive moderate significant correlation to red muscle fibber abundance and tissue. In relation to number of feeding days we found significant moderate correlations to white and red muscle fibber abundance. Correlation coefficients between muscle fibre diameter and live weight, daily gain, number of feeding days and food intake/kg gain were found predominantly weak, and non significant.


Keywords


pig; MLD; muscle fibres; feeding indicators

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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Faculty of Animal Science and Biotechnologies
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