QUANTITATIVE MORPHOMETRY ANALYSIS OF THE RAT EPIDIDYMIS AFTER A PERORAL ADMINISTRATION OF NICKEL

L. BÁBIKOVÁ, R. TOMAN, S. HLUCHÝ, P. MASSÁNYI

Abstract


The aim of the study was to evaluate the changes of rat epididymis after a long term intake of nickel chloride using morphometry methods. The nickel was administered in a daily dose of 100 mg.l-1 NiCl2 in drinking water to male rats during 3 months. The males were housed individually in a plastic cages. Ten males served as an untreated control group without nickel treatment. At the end of the experimental period (3 months), the animals from nickel–treated group and control group were killed and the samples of the epididymes were taken for morphometry evaluation. Three months after nickel treatment, no significant decrease in interstitial tissue volume from 61.23 ± 15.29 % to 59.44 ± 16.91 % in the epididymis of Ni- treated rats was observed. The tubule epithelium in nickeltreated group increased insignificantly to 25.97 ± 11.75 % and the tubule lumen volume to 14.58 ± 7.21 % in comparison with the control group (24.17 ± 11.08 % and 14.52 ± 8.40 %, respectively). The diameter of the epididymal tubule significantly (P<0.0001) increased from 168.31 ± 53.96 % to 218.85 ± 96.97 %. A significant (P<0.0001) decrease in the epithelium height from 33.55 ± 9.90 % to 28.18 ± 9.75 % was also noted. The findings show the slight effect of peroral nickel on the epididymal structures indicating the possibility of the male fertility depression caused by nickel.

 


Keywords


epididymis; nickel; rat; morphometry; structure

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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
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FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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