DIAGNOSTICATION OF MALIGNANT HYPERTHERMIA IN SWINE

MARIA ADINA MANEA, STELIANA KEVORKIAN, S.E. GEORGESCU, ANCA DINISCHIOTU, MARIETA COSTACHE

Abstract


Malignant hyperthermia (MH) causes neurological, liver, and kidney damage and death in humans and major economic losses in the swine industry. A single point mutation in the gene for the skeletal muscle ryanodine receptor (Ryrl) was found to be correlated with MH in major breeds of swine. This mutation generates the production of an abnormal protein which alters the structure and function of the Ca2+ release channel of skeletal muscle sarcoplasmic reticulum (ryanodine receptor). Because the mutation destroys the recognition site for the restriction endonuclease HhaI, it was possible to develop a PCR-RFLP method for MH diagnostication in all swine breeds. Our objective was to identify the normal homozygous, heterozygous carriers and affected homozygous swine for MH trait, using this method. Results suggest that the genetic test will be useful in identifying heterozygous pigs for the MH trait.


Keywords


swine; malignant hyperthermia; PCR-RFLP; diagnostication

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Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Faculty of Animal Science and Biotechnologies
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
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