GENOTYPING OF β-LACTOGLOBULIN GENE IN KARAKUL SHEEP BREED

STELIANA KEVORKIAN, MARIA ADINA MANEA, S.E. GEORGESCU, ANCA DINISCHIOTU, MARIETA COSTACHE

Abstract


β-lactoglobulin is the major milk whey protein in the ruminants. Studies have indicated that this protein is polymorphic in the many breeds of sheep. This is the result of a single base pair substitution in the β-lactoglobulin gene. The genetic variants, A (tyrosine) and B (histidine), differ at the amino acid position 20. The nucleotide mutation disrupts an RsaI site and can be detected by PCR-RFLP analyses. We included in this study 52 animals from Karakul breed. The Karakul may be the oldest breed of domesticated sheep. In Romania it is raised especially for pelts. The aim of this work was to analyze the genotype distribution of β- lactoglobulin in Karakul sheep.


Keywords


β-lactoglobulin; sheep; PCR-RFLP; genotyping

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Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Faculty of Animal Science and Biotechnologies
300645, Timişoara, Calea Aradului 119, Romania

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