ANALYSIS OF POLYMORPHISM OF CAST GENE AND CLPG GENE IN SHEEP BY PCR-RFLP METHOD

M. GÁBOR, ANNA TRAKOVICKÁ, MARTINA MILUCHOVÁ

Abstract


The work was oriented to identification of calpastatin gene polymorphism and callipyge gene polymorphism and analysis of genotype structure in population of sheep kept in Slovakia. The material involved 96 sheep of breeds tsigai, improved valachian, east friesian, lacaune and crossbreds lacaune and tsigai . Genomic DNA was isolated by salting out method and used in order to estimate calpastatin and callipyge genotypes by means of PCR-RFLP method. The PCR products were digested with MspI restriction enzyme for calpastatin gene and FaqI restriction enzyme for callipyge gene. In the total population of sheep was detected homozygous genotype MM – 0.87, heterozygous genotype MN – 0.13 for calpastatin gene. Homozygous genotype NN has not been observed. For the callipyge gene was detected homozygous genotype AA – 1.00 only. The heterozygous genotype AG and homozygous genotype GG was not detected.


Keywords


sheep; PCR-RFLP; calpastatin gene; callipyge gene

Full Text:

PDF

Refbacks

  • There are currently no refbacks.


Copyright (c)


Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
_________________________________________________________________

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Faculty of Animal Science and Biotechnologies
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110