Effects of Bioprocess Parameters on Production of Cellulase using Miscanthus as Substrate

Adrian Trulea, Teodor Vintilă, Georgeta Pop, Monica Dragomirescu

Abstract


A number of fungi were isolated abroad and screened for cellulolytic potential. In this work we studied cellulase production with several fungi strains, using Miscanthus giganteus as substrate. An indigenous strain of T. viride was selected and the kinetics of cellulase production is reported. Product formation parameters of different types of cellulases indicate that the studied strain of T. viride is capable of producing important levels of cellulases when grown on Mandels medium with Miscanthus biomass as carbon source. Furthermore, it was observed that production of endoglucanase reaches its maximum during exponential phase of growth, while exoglucanase during the stationary phase. Enzyme production by solid-state fermentation was also investigated and found to be more efficient than liquid state fermentation. High production of cellulase was noted at the following parameters for liquid cultures: 4% Miscanthus giganteus biomass, 5% inoculum, 180 r.p.m. agitation, pH 5; and 60% humidity in the case of solid state fermentation. Comparing the productions of cellulases in the fermentations carried out in this study, data indicate Miscanthus as ideal substrate for cellulase biosynthesis with fungi.

Keywords


cellulase; fermentation; fungi; Miscanthus

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Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110